• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

2011 Fiscal Year Final Research Report

Site-specific incorporation of non-natural amino acids into proteins by using pyrrolysyl-tRNA synthetase

Research Project

  • PDF
Project/Area Number 21550167
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Chemistry related to living body
Research InstitutionThe Institute of Physical and Chemical Research

Principal Investigator

YANAGISAWA Tatsuo  独立行政法人理化学研究所, システム研究チーム, 研究員 (10450420)

Project Period (FY) 2009 – 2011
Keywordsバイオテクノロジー / 非天然型アミノ酸 / タンパク質 / クロスリンカー / ケミカルバイオロジー / 翻訳 / ピロリジン / アミノアシルtRNA合成酵素
Research Abstract

Site-specific incorporation of N^ε-(p-trifluoreomethyldiazirinylbenzyloxycarbonyl) lysine(TmdZLys), a new lysine derivative with a diazirine photo-crosslinker, into proteins has been achieved by the pyrrolysyl-tRNA synthetases(PylRS)/tRNA^<Pyl> system. The incorporated TmdZLys enabled wide-range photo-crosslinking with target proteins. This system is useful for probing protein interaction networks in living cells.

  • Research Products

    (6 results)

All 2012 2011 2010 2009 Other

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (3 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article]2012

    • Author(s)
      Yanagisawa, T., Hino, N., Iraha, F., Mukai, T., Sakamoto, K., Yokoyama, S.
    • Journal Title

      Mol. BioSyst

      Volume: 8 Pages: 1131-1135

    • Peer Reviewed
  • [Journal Article]2011

    • Author(s)
      Mukai, T., Yanagisawa, T., Ohtake, K., Wakamori, M., Adachi, J., Hino, N., Sato, A., Kobayashi, T., Hayashi, A., Shirouzu, M., Umehara, T., Yokoyama, S., Sakamoto, K.
    • Journal Title

      Biochem. Biophys. Res. Commun

      Volume: 411 Pages: 757-761

    • Peer Reviewed
  • [Presentation] 終止コドンUAGがセンス・コドンに再定義された大腸菌株を用いた非天然型アミノ酸のタンパク質への導入2011

    • Author(s)
      坂本健作、安達仁朗、若森昌聡、白水美香子、梅原崇史、柳沢達男、向井崇人、横山茂之
    • Organizer
      第6回無細胞生命科学研究会
    • Place of Presentation
      兵庫
    • Year and Date
      2011-11-16
  • [Presentation] 大腸菌、昆虫細胞、動物細胞を用いた非天然型アミノ酸のタンパク質への部位特異的導入法の開発2010

    • Author(s)
      坂本健作、向井崇人、大竹和正、伊良波史枝、林明子、佐藤文、樋野展正、柳沢達男、脇山素明、横山茂之
    • Organizer
      第10回日本蛋白質科学会年会
    • Place of Presentation
      札幌
    • Year and Date
      2010-06-17
  • [Presentation] 翻訳後修飾アミノ酸のタンパク質への部位特異的導入2009

    • Author(s)
      坂本健作、小林隆嗣、柳沢達男、横山茂之
    • Organizer
      第4回無細胞生命科学研究会
    • Place of Presentation
      岐阜
    • Year and Date
      2009-11-16
  • [Patent(Industrial Property Rights)] 新規非天然型アミノ酸とその応用に関する研究: N(epsilin)-(p-trifluoromethyldiazirinylbenzylo xycarbonyl) lysine

    • Inventor(s)
      Yokoyama, S., Sakamoto, K., Yanagisawa, T., Hino, N., F. Iraha
    • Industrial Property Rights Holder
      Yokoyama, S., Sakamoto, K., Yanagisawa, T., Hino, N., F. Iraha
    • Industrial Property Number
      特願、2011-253307

URL: 

Published: 2013-07-31  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi