2011 Fiscal Year Final Research Report
Mechanosensing in vascular endothelial cells and its response to the physiological stress environment
| Project/Area Number |
21560195
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Fluid engineering
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| Research Institution | 防衛大学校 |
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Principal Investigator |
TADA Shigeru 防衛大学校, 応用科学群, 教授 (70251650)
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| Co-Investigator(Kenkyū-buntansha) |
KUDOH Susumu 芝浦工業大学, 工学部, 教授 (70306926)
ICHIMIYA Kohichi 山梨大学, 医学工学総合研究部, 非常勤講師 (30037923)
TORIYAMA Kohji 山梨大学, 医学工学総合研究部, 助教 (50313789)
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| Project Period (FY) |
2009 – 2011
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| Keywords | バイオ流体力学 |
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| Research Abstract |
The present study demonstrated that : I) Exposure of cultured bovine aortic endothelial cells(BAEC) to fluid flow resulted in nitric oxide(NO) production and up-regulation of intracellular calcium ion concentration([Ca2+] i), monitored by fluorescent dyes of DAF2-DA and Rhod2-DA, respectively using a confocal laser scanning microscope, II) A two-dimensional(2-D) numerical simulation of a convective-diffusive transport of LDL in the artery wall coupled with the wall shear stress gradient(WSSG) dependent LDL consumption of smooth muscle cells(SMCs) resulted in a strong dependence of LDL uptake of SMCs on WSSG for cells right beneath the fenestral pores, and III) Construction of a three-dimensional models of bifurcations with two different bifurcation angles mimicking the real bifurcation anatomy to perform simulations of steady blood flow under the wall boundary condition of a constant oxygen concentration, resulted in the significant alternation of the distribution and the magnitude of the oxygen wall flux separation at the outer common-internal carotid wall, depending strongly on the bifurcation angle.
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