2012 Fiscal Year Final Research Report
Analysis of regulations of septin modifications in cytokinesis
| Project/Area Number |
21570003
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Genetics/Genome dynamics
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| Research Institution | Gakushuin University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
UESONO Yukifumi 東京大学, 大学院・理学研究科, 助教 (30251408)
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| Project Period (FY) |
2009 – 2012
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| Keywords | 分子遺伝 / 細胞周期 / 細胞質分裂 / SUMO化 / ユビキチン化 / セプチン |
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| Research Abstract |
Budding yeast Cdc3 is a component of septin rings which localize at the neck region andis essential for cytokinesis. Cdc3 was transiently sumoylated by Siz1, a SUMO ligase onlyduring M-phase. Cdc3 was ubiquitinated in Rsp5-dependent way, but the PY-motif of Cdc3was not required for the ubiquitination. We examined the phenotypes of various mutantswhen Siz1-septin or SUMO-septin fusion proteins were over-expressed. Results suggestedthat the septin sumoylation induces the depolymerization of septin rings, and that thesumolylated Siz1 was ubiquitinated by Slx8/Slx5 ubiquitin ligase for its degradation.
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