Understanding the novel interplay between telomere and helicase in chromosome maintenance

2011 Fiscal Year Final Research Report

• Project/Area Number: 21570006
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Genetics/Genome dynamics
• Research Institution: Hiroshima University
• Principal Investigator: MASARU Ueno 広島大学, 大学院・先端物質科学研究科, 准教授 (90293597)
• Project Period (FY): 2009 – 2011
• Keywords: テロメア / 分裂酵母 / RecQヘリケース

Research Abstract

The double mutants between fission yeast telomere binding protein Pot1 and Rqh1 helicase and the double mutant between fission yeast telomerase Trt1 and Rqh1 helicase are sensitive to microtubule drug TBZ. We found that the reason for the TBZ sensitivity is due to the accumulation of recombination intermediates at telomere in M phase, which inhibits chromosome segregation.

Research Products

• [Journal Article] Double mutant between fission yeast telomerase and RecQ helicase is sensitive to anti-microtubule drug (2012)
  • Author(s): S. Ukimori, N. Kawabata, H. Shimada, Ryota Imano, K. Takahashi, M. Yukawa, E. Tsuchiya, and M. Ueno
  • Journal Title: Biosci. Biotechnol. Biochem Volume: 76 Pages: 264-269
  • Peer Reviewed
• [Journal Article] Fission yeast Pot1 and RecQ helicase are required for efficient chromosome segregation (2011)
  • Author(s): K. Takahashi, R. Imano, T. Kibe, H. Seimiya, Y. Muramatsu, N. Kawabata, G. Tanaka, Y. Matsumoto, T. Hiromoto, Y. Koizumi, N. Nakazawa, M. Yanagida, M. Yukawa, E. Tsuchiya, M. Ueno
  • Journal Title: Mol. Cell. Biol Volume: 31 Pages: 495-506
  • Peer Reviewed
• [Journal Article] Expression of Mutant RPA in Human Cancer Cells Causes Telomere Shortening (2010)
  • Author(s): Y. Kobayashi, K. Sato, T. Kibe, H. Seimiya, A. Nakamura, M. Yukawa, E. Tsuchiya, and M. Ueno
  • Journal Title: Biosci. Biotechnol. Biochem Volume: 74 Pages: 382-385
  • Peer Reviewed
• [Journal Article] Roles of DNA repair proteins in telomere maintenance (2010)
  • Author(s): M. Ueno Biosci
  • Journal Title: Biotechnol. Biochem Volume: 74 Pages: 1-6
  • Peer Reviewed
• [Presentation] Dysfunctional telomere activatesboth DNA damage checkpoint andspindle checkpoint in fission yeast (2011)
  • Author(s): S. pombe M. Ueno
  • Organizer: The 5th International Workshop onCell Regulations in Division & Arrest
  • Place of Presentation: Okinawa, Japan
  • Year and Date: 2011-04-04
• [Presentation] Entangled telomere activates both DNA damage checkpoint and spindlecheckpoint (2011)
  • Author(s): M. Ueno
  • Organizer: The 6th international fission yeastmeeting
  • Place of Presentation: Boston, U. S. A.
  • Year and Date: 2011-01-14
• [Presentation] Roles of Fission Yeast Pot1 and RecQHelicases in Telomere Protection (2011)
  • Author(s): M. Ueno
  • Organizer: Cold Spring Haber Laboratory MeetingTelomere and Telomerase
  • Place of Presentation: New York, U. S. A.
  • Year and Date: 2011-01-05
• [Presentation] Role of RecQ Helicase at Dysfunctional Telomere (2009)
  • Author(s): M. Ueno
  • Organizer: Cold Spring Haber Laboratory Meeting Telomere and Telomerase
  • Place of Presentation: New York, U. S. A.
  • Year and Date: 20090229
• [Presentation] Novel Roles of Telomere Proteins and Rec Q Helicases in Genome Stability (2009)
  • Author(s): M. Ueno
  • Organizer: The 5th international fission yeastmeeting
  • Place of Presentation: Tolyo, Japan
  • Year and Date: 2009-03-25
• [Presentation] Roles of Pot1 and RecQ Helicases in Genome Stability (2009)
  • Author(s): M. Ueno
  • Organizer: Switzerland-Japan Meeting on the Molecular Mechanisms Regulating Chromosome Dynamics and Genome Stability
  • Place of Presentation: Villars, Switzerland
  • Year and Date: 2009-03-08
• [Book] Roles of DNA Repair Proteins in TelomereMaintenance (2011)
  • Author(s): タイトルDNA Repair
  • Total Pages: 597-612
  • Publisher: InTech, ISBN 978-953-307-697-3編集InnaKruman 2011