2011 Fiscal Year Final Research Report
Visualization of the site of phospholipid biosynthesis in Arabidopsis roots under low temperature conditions
| Project/Area Number |
21570034
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Saitama University |
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Principal Investigator |
NISHIDA Ikuo 埼玉大学, 大学院・理工学研究科, 教授 (40189288)
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| Project Period (FY) |
2009 – 2011
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| Keywords | ホスファチジルコリン / ホスファチジルエタノールアミン / 低温応答 / リン脂質合成 / 根の伸長 / 表皮細胞の内膜構造 / CDP-コリン合成 / ホスファチジルセリン |
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| Research Abstract |
WS, cct2, cct1-1and cct1-1 cct2 plants exhibited CCT activity in descending order. cct1-1 cct2 seedlings were unable to elongate roots at 2. Root epidermal cells showed ER-Tracker fluorescence comparably in WS, cct2 and cct1-1 seedlings at ambient temperature and expandedly at 2 or under salt stresses. In contrast, endomembrane structures developed less expensively in cct1-1 cct2 seedlings at ambient temperature and disappeared in cold-or salt-stressed seedlings. sGFP-CCT1 and sGFP-CCT2 fluorescence was associated with ER-Tracker fluorescence in transgenic Arabidopsis roots. These results suggest that functional CCTs are required for stress-induced ER development and cell elongation in Arabidopsis roots.
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[Presentation] The effects of pect1-4mutation on the lipid composition and the respiration activity of isolated mitochondria2010
Author(s)
Yu, Y., Kawai, H., Kawamoto, M., Mizoi, J., Fujiki, Y., Nishida, I.
Organizer
第51回日本植物生理学会年会
Place of Presentation
熊本
Year and Date
20100318-21
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