2011 Fiscal Year Final Research Report
In vitro studies on the mechanism underlying temperature compensation of circadian period
| Project/Area Number |
21570037
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant molecular biology/Plant physiology
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| Research Institution | Nagoya University |
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Principal Investigator |
OHKAWA Taeko (NISHIWAKI Taeko) 名古屋大学, 理学研究科, 特任講師 (30432230)
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| Project Period (FY) |
2009 – 2011
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| Keywords | シアノバクテリア / 概日時計 / リン酸化 / 脱リン酸化 / ATP |
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| Research Abstract |
Circadian phosphorylation rhythm of cyanobacterial clock protein KaiC can be reconstituted in vitro by mixing KaiC with KaiA and KaiB in the presence of ATP. The period of the rhythm is compensated for the changes in the ambient temperature. In this study, we clarified that the phosphorylation rhythm of KaiC results from repeating synthesis and degradation of ATP. The higher the temperature, the equilibrium was more favorable to ATP formation. These results can serve as important clues to elucidate the mechanism for temperature compensation of circadian period.
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