Molecular mechanism of seed maturation, dormancy and germination using viviparous mutants

2011 Fiscal Year Final Research Report

• Project/Area Number: 21570054
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant molecular biology/Plant physiology
• Research Institution: Japan International Research Center for Agricultural Sciences
• Principal Investigator: NAKASHIMA Kazuo 独立行政法人国際農林水産業研究センター, 生物資源・利用領域, 主任研究員 (90343772)
• Project Period (FY): 2009 – 2011
• Keywords: 穂発芽 / アブシシン酸 / タンパク質リン酸化酵素 / SnRK2 / bZIP型転写因子

Research Abstract

Abscisic acid(ABA) is an important phytohormone regulating various physiological processes including seed maturation, dormancy and germination. SRK2D, SRK2E and SRK2I are redundant ABA-activated SNF1-related protein kinases 2(SnRK2s) in Arabidopsis thaliana. The triple mutant of the SnRK2 protein kinases(srk2d srk2e srk2i) showed viviparous germination. It was sensitive to desiccation and showed severe growth defects during seed development. It exhibited a loss of dormancy, strong ABA-insensitivity, and elevated seed ABA content relative to wild-type seeds. The triple mutant had greatly reduced phosphorylation activity in in-gel kinase experiments using bZIP transcription factors including ABI5.Microarray experiments indicate that SRK2D, SRK2E and SRK2I protein kinases involved in ABA signaling are essential for the control of seed development, dormancy and germination through the extensive control of gene expression.

Research Products

• [Journal Article] NAC transcription factors in plant abiotic stress responses (2012)
  • Author(s): Nakashima K, Takasaki H, Mizoi J, Shinozaki K, Yamaguchi-Shinozaki K
  • Journal Title: Biochim Biophys Acta Volume: 1819 Pages: 97-103
  • DOI: DOI:10.1016/j.bbagrm.2011.10.005
  • Peer Reviewed
• [Journal Article] Molecular basis of the core regulatory network in ABA responses : sensing, signaling and transport (2010)
  • Author(s): Umezawa T, Nakashima K, Miyakawa T, Kuromori T, Tanokura M, Shinozaki K, Yamaguchi-Shinozaki K
  • Journal Title: Plant Cell Physiol Volume: 51 Pages: 1821-39
  • DOI: DOI:10.1093/pcp/pcq156
  • Peer Reviewed
• [Journal Article] Three Arabidopsis SnRK2 protein kinases, SRK2D/SnRK2.2, SRK2E/SnRK2.6/OST1 and SRK2I/SnRK2.3, involved in ABA signaling are essential for the control of seed development and dormancy (2009)
  • Author(s): Nakashima K, Fujita Y, Kanamori N, Katagiri T, Umezawa T, Kidokoro S, Maruyama K, Yoshida T, Ishiyama K, Kobayashi M, Shinozaki K, Yamaguchi-Shinozaki K.
  • Journal Title: Plant Cell Physiol Volume: 50 Pages: 1345-63
  • DOI: DOI:10.1093/pcp/pcp083
  • Peer Reviewed
• [Journal Article] Three SnRK2 protein kinases are the main positive regulators of abscisic acid signaling in response to water stress in Arabidopsis (2009)
  • Author(s): Fujita Y, Nakashima K, Yoshida T, Katagiri T, Kidokoro S, Kanamori N, Umezawa T, Fujita M, Maruyama K, Ishiyama K, Kobayashi M, Nakasone S, Yamada K, Ito T, Shinozaki K, Yamaguchi-Shinozaki K
  • Journal Title: Plant Cell Physiol Volume: 50 Pages: 2123-32
  • DOI: DOI:10.1093/pcp/pcp147
  • Peer Reviewed
• [Presentation] シロイヌナズナとイネにおけるABA受容・シグナル伝達コンポーネント群の乾燥応答性遺伝子発現の解析 (2012)
  • Author(s): 中島一雄、藤田泰成、圓山恭之進、篠崎一雄、篠崎和子
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学(京都市)
  • Year and Date: 2012-03-18
• [Presentation] シロイヌナズナ、ダイズ、イネの水ストレス応答におけるAREB-SnRK2経路の役割 (2012)
  • Author(s): 藤田泰成、吉田拓也、Tory Chhun、関田佐知子、Nang Myint Phyu Sin Htwe、中島一雄、藤田美紀、戸高大輔、城所聡、溝井順哉、篠崎一雄、篠崎和子
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都産業大学(京都市)
  • Year and Date: 2012-03-18
• [Presentation] Role of AREB-SnRK2 pathway in response to osmotic stress (2011)
  • Author(s): Fujita, Y., Yoshida, T., Chhun, T., Nakashima, K., Fujita, M., Kidokoro, S., Mizoi, J., Shinozaki, K., Yamaguchi-Shinozaki, K.
  • Organizer: 4th International Workshop on Plant Abiotic Stress : From Systems Biology To Sustainable Agriculture
  • Place of Presentation: Limassol, Cyprus
  • Year and Date: 20111117-19
• [Presentation] Plant regulatory systems in drought stress responses (2011)
  • Author(s): Shinozaki, K., Yamaguchi-Shinozaki, K., Kuromori, T., Umezawa, T., Nakashima, K., Fujita, Y.
  • Organizer: 4th International Workshop on Plant Abiotic Stress : From Systems Biology To Sustainable Agriculture
  • Place of Presentation: Limassol, Cyprus
  • Year and Date: 20111117-19
• [Presentation] Three SnRK2 protein kinases involved in ABA-signaling function for the control of seed dormancy and abiotic stress tolerance in Arabidopsis (2011)
  • Author(s): Nakashima, K., Fujita, Y., Maruyama, K., Shinozaki, K., Yamaguchi-Shinozaki, K.
  • Organizer: 22nd International Conference on Arabidopsis Research
  • Place of Presentation: Wisconsin, USA
  • Year and Date: 20110622-25
• [Presentation] シロイヌナズナのABAシグナリングに関わる3種のSnRK2タンパク質リン酸化酵素は種子の成熟と環境ストレス耐性を制御する (2011)
  • Author(s): 中島一雄、藤田泰成、圓山恭之進、篠崎一雄、篠崎和子
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 東北大学(仙台市)
  • Year and Date: 2011-03-22
• [Presentation] シロイヌナズナとイネの水ストレス応答におけるAREB/ABF-SnRK2経路の役割 (2011)
  • Author(s): 藤田泰成、吉田拓也、Tory Chhun、中島一雄、藤田美紀、城所聡、溝井順哉、篠崎一雄、篠崎和子
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 東北大学(仙台市)
  • Year and Date: 2011-03-22
• [Presentation] Three SnRK2 protein kinases are the main positive regulators of ABA signaling in seed development and dormancy in Arabidopsis (2010)
  • Author(s): Nakashima, K., Fujita, Y., Kanamori, N., Katagiri, T., Umezawa, T., Kidokoro, S., Maruyama, K., Yoshida, T., Ishiyama, K., Kobayashi, M., Shinozaki, K., Yamaguchi-Shinozaki, K.
  • Organizer: The 21st International Conference on Arabidopsis Research(ICAR 2010)
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 20100606-10
• [Presentation] AREB/ABF-SnRK2 pathway involved in ABA signaling in response to water stress (2010)
  • Author(s): Fujita, Y., Yoshida, T., Nakashima, K., Nakasone, S., Chhun, T., Kidokoro, S., Fujita, M., Maruyama, K., Mizoi, J., Shinozaki, K., Yamaguchi-Shinozaki, K.
  • Organizer: The 21st International Conference on Arabidopsis Research(ICAR 2010)
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 20100606-10
• [Presentation] シロイヌナズナのABAシグナリングに関わるSnRK2タンパク質リン酸化酵素SRK2D/SnRK2.2、SRK2E/SnRK2.6/OST1、SRK2I/SnRK2.3は種子成熟・発芽過程で必須である (2010)
  • Author(s): 中島一雄、藤田泰成、金森紀仁、片桐健、梅澤泰史、城所聡、圓山恭之進、吉田拓也、石山賀奈子、小林正智、篠崎一雄、篠崎和子
  • Organizer: 第51回日本植物生理学会年会
  • Place of Presentation: 熊本大学(熊本市)
  • Year and Date: 2010-03-21
• [Presentation] 乾燥ストレス時のABAシグナル伝達系におけるAREB/ABF-SnRK2経路の役割 (2010)
  • Author(s): 藤田泰成、吉田拓也、Tory Chhun、佐山博子、中島一雄、城所聡、藤田美紀、圓山恭之進、溝井順哉、篠崎一雄、篠崎和子
  • Organizer: 第51回日本植物生理学会年
  • Place of Presentation: 熊本大学(熊本市)
  • Year and Date: 2010-03-19
• [Presentation] シロイヌナズナの乾燥ストレス応答に関わるAREB-SnRK2経路のアブシシン酸シグナル伝達系における役割 (2009)
  • Author(s): 藤田泰成、吉田拓也、中島一雄、城所聡、藤田美紀、圓山恭之進、仲宗根尚子、石山賀奈子、小林正智、篠崎一雄、篠崎和子
  • Organizer: 第32回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 20091209-12
• [Presentation] Three Arabidopsis ABA-activated SnRK2 protein kinases, SRK2D/SnRK2.2, SRK2E/SnRK2.6/OST1, and SRK2I/SnRK2.3 are essential in ABA-signaling to control seed development and dormancy (2009)
  • Author(s): Nakashima, K., Fujita, Y., Kanamori, N., Katagiri, T., Umezawa, T., Kidokoro, S., Maruyama, K., Yoshida, T., Ishiyama, K., Kobayashi, M., Shinozaki, K. and Yamaguchi-Shinozaki, K.
  • Organizer: The 9th International Plant Molecular Biology Congress
  • Place of Presentation: St. Louis, USA
  • Year and Date: 20091025-30
• [Book] Promoters and transcription factors in abiotic stress-responsive gene expression. Pareek, A., Sopor, S. K., and Bohnert, H. J.(eds) Abiotic Stress Adaptation in Plants : Physiological, Molecular and Genomic Foundation (2010)
  • Author(s): Nakashima, K. and Yamaguchi-Shinozaki, K.
  • Total Pages: 199-216
  • Publisher: Springer-Verlag Berlin
• [Remarks]
  • URL: http://www.jircas.affrc.go.jp/kankoubutsu/seika/seika2009/2009_08.html
• [Remarks]
  • URL: http://www.jircas.affrc.go.jp/kankoubutsu/highlight/highlights2009/2009_08.html