Osmoregulation in elephant fish : introduction of a new model animal for cartilaginous fish research

2011 Fiscal Year Final Research Report

• Project/Area Number: 21570061
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphology/Structure
• Research Institution: The University of Tokyo
• Principal Investigator: HYODO Susumu 東京大学, 大気海洋研究所, 准教授 (40222244)
• Project Period (FY): 2009 – 2011
• Keywords: 軟骨魚類 / 尿素 / ゾウギンザメ / 腎臓 / 初期発生 / ホルモン受容体

Research Abstract

The aim of this study is to investigate adaptive, reproductive and developmental physiologies of the cartilaginous fish by using elephant fish as a model organism, for which the public genome database is available. We have proposed a urea reabsorption model in the renal nephron by mapping of various transporting proteins for urea, water and ions. In the present study, we also found contribution of the yolk-sac membrane to body fluid regulation in the early developmental stages, and a novel neurohypophysial hormone receptor that is likely to be involved in the maintenance of homeostasis.

Research Products

• [Journal Article] The fifth neurohypophysial hormone receptor(V4R) is structurally related to the V2-type receptor but functionally similar to V1-and V3-type receptors (2012)
  • Author(s): Yamaguchi, Y., Kaiya, H., Konno, N., Iwata, E., Miyazato, M., Uchiyama, M., Bell, J. D., Toop, T., Donald, J. A., Brenner, S., Venkatesh, B., Hyodo, S.
  • Journal Title: Gen. Comp. Endocrinol Volume: (in press)
  • Peer Reviewed
• [Journal Article] Morphological and functional characterization of a novel Na+/K+-ATPaseimmunoreactive, follicle-like structure on the gill septum of Japanese banded houndshark (2012)
  • Author(s): Takabe, S., Teranishi, K., Takaki, S., Kusakabe, M., Hirose, S., Kaneko, T., Hyodo, S.
  • Journal Title: Cell Tissue Res Volume: 348 Pages: 141-153
  • DOI: DOI:10.1007/s00441-012-1344-5
  • Peer Reviewed
• [Journal Article] Hepatic and extrahepatic distribution of ornithine urea cycle enzymes in holocephalan elephant fish (2012)
  • Author(s): Takagi, W., Kajimura, M., Bell, J. D., Toop, T., Donald, J. A., Hyodo, S.
  • Journal Title: Comp. Biochem. Physiol. B Volume: 161 Pages: 331-340
  • DOI: doi:10.1016/j.cbpb.2011.12.006
  • Peer Reviewed
• [Journal Article] 軟骨魚類の生理学研究:体液調節を中心にライフサイクルを追う (2010)
  • Author(s): 兵藤晋
  • Journal Title: 板鰓類研究会報 Volume: 46 Pages: 1-7
• [Journal Article] Vasotocin/V2-type receptor/aquaporin axis exists in African lungfish kidney but is functional only in terrestrial condition (2010)
  • Author(s): Konno, N., Hyodo, S., Yamaguchi, Y., Matsuda, K., Uchiyama, M.
  • Journal Title: Endocrinology Volume: 151 Pages: 1089-1096
  • DOI: doi:10.1210/en.2009-1070
  • Peer Reviewed
• [Journal Article] A new method to calibrate attachment angles of data loggers in swimming sharks. Eurasip J. Advance in Signal (2010)
  • Author(s): Kawatsu, S., Sato, K., Watanabe, Y., Hyodo, S., Breves, J. P., Fox, B. K., Grau, E. G., Miyazaki, N.
  • Journal Title: Processing Pages: 732586
  • DOI: doi:10.1155/2010/732586
  • Peer Reviewed
• [Journal Article] Rhesus glycoprotein p2(Rhp2) is a novel member of the Rh family of ammonia transporters highly expressed in shark kidney (2010)
  • Author(s): Nakada, T., Westhoff, C. M., Yamaguchi, Y., Hyodo, S., Li, X., Muro, T., Kato, A., Nakamura, N., Hirose, S.
  • Journal Title: J. Biol. Chem Volume: 285 Pages: 2653-2664
  • DOI: doi:10.1074/jbc.M109.052068
  • Peer Reviewed
• [Journal Article] Multiple urea transporter proteins in the kidney of holocephalan elephant fish. Comp (2009)
  • Author(s): Kakumura, K., Watanabe, S., Bell, J. D., Donald, J. A., Toop, T., Kaneko, T., Hyodo, S.
  • Journal Title: Biochem. Physiol. B Volume: 154 Pages: 239-247
  • DOI: doi:10.1016/j.cbpb.
  • Peer Reviewed
• [Journal Article] Subcellular distribution of urea transporter in the collecting tubule of shark kidney is dependent on environmental salinity (2009)
  • Author(s): Yamaguchi, Y., Takaki, S., Hyodo, S.
  • Journal Title: J. Exp. Zool Volume: 311A Pages: 705-718
  • DOI: DOI:10.1002/jez.558
  • Peer Reviewed
• [Journal Article] African lungfish possess an arginine vasotocin receptor homologous to the tetrapod V2-type receptor (2009)
  • Author(s): Konno, N., Hyodo, S., Yamaguchi, Y., Kaiya, H., Miyazato, M., Matsuda, K., Uchiyama, M.
  • Journal Title: J. Exp. Biol Volume: 212 Pages: 2183-2193
  • DOI: doi:10.1242/jeb.029322
  • Peer Reviewed
• [Presentation] 卵生軟骨魚類の発生過程における浸透圧調節 (2012)
  • Author(s): 高木亙
  • Organizer: 平成24年度日本水産学会
  • Place of Presentation: 東京海洋大学
  • Year and Date: 2012-03-28
• [Presentation] ゾウギンザメ腎臓における尿素輸送体および尿素輸送関連分子の局在と機能 (2012)
  • Author(s): 角村佳吾
  • Organizer: 平成24年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学
  • Year and Date: 2012-03-28
• [Presentation] 脊椎動物における新規バソプレシン/オキシトシン受容体、V1cRファミリーの発見 (2012)
  • Author(s): 山口陽子
  • Organizer: 第22回バソプレシン研究会
  • Place of Presentation: 慶應義塾大学病院
  • Year and Date: 2012-01-07
• [Presentation] 脊椎動物における新規神経葉ホルモン受容体V1cRファミリーの発見-軟骨魚類の研究から- (2011)
  • Author(s): 山口陽子
  • Organizer: 第36回日本比較内分泌学会、シンポジウム
  • Place of Presentation: 都道府県会館
  • Year and Date: 2011-11-23
• [Presentation] Ornithine urea cycle enzymes inholocephalan elephant fish (2011)
  • Author(s): Takagi, W.
  • Organizer: International Congress of Comparative Physiology and Biochemistry
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2011-06-04
• [Presentation] Morphological and functional characteristics of a novel Na+/K+-ATPase-immunoreactive, follicle-like structure in the gill septum of Japanese banded hound shark (2011)
  • Author(s): Takabe, S.
  • Organizer: International Congress of Comparative Physiology and Biochemistry
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2011-06-02
• [Presentation] Identification and mapping of urea-and ion-transporting molecules in the kidney of cartilaginous fish (2011)
  • Author(s): Hyodo, S.
  • Organizer: International Congress of Comparative Physiology and Biochemistry
  • Place of Presentation: 名古屋国際会議場
  • Year and Date: 2011-06-01
• [Presentation] サメ、エイ、ギンザメ:体液調節を中心にそのライフサイクルを追う (2010)
  • Author(s): 兵藤晋
  • Organizer: 第11回日本比較三学会合同シンポジウム
  • Place of Presentation: 静岡グランシップ
  • Year and Date: 2010-11-20
• [Presentation] ドチザメにおけるカルバミルリン酸合成酵素IIIの分布と環境塩分変化の影響 (2010)
  • Author(s): 山口陽子
  • Organizer: 日本動物学会第81回大会
  • Place of Presentation: 東京大学教養学部
  • Year and Date: 2010-09-23
• [Presentation] サメの鰓は塩分排出器官か (2009)
  • Author(s): 兵藤晋
  • Organizer: 日本比較内分泌学会第34回大会
  • Place of Presentation: 千里ライフサイエンスセンター
  • Year and Date: 2009-10-23
• [Presentation] ゾウギンザメは軟骨魚類研究のブレークスルーをもたらすか (2009)
  • Author(s): 兵藤晋
  • Organizer: 日本動物学会第80回大会
  • Place of Presentation: 静岡グランシップ
  • Year and Date: 2009-09-18
• [Remarks]
  • URL: http://physiol.ori.u-tokyo.ac.jp/seiri/hyodo/index.html