Ultrastructural analysis of newly indentified membrane structure in autophagy

2011 Fiscal Year Final Research Report

• Project/Area Number: 21570069
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Morphology/Structure
• Research Institution: Kogakuin University (2010-2011); Japan Women's University (2009)
• Principal Investigator: BABA Misuzu 工学院大学, 総合研究所, 研究員 (80435528)
• Co-Investigator(Renkei-kenkyūsha): BABA Norio 工学院大学, 情報工学部, 教授 (80164896)
• Project Period (FY): 2009 – 2011
• Keywords: 免疫電子顕微鏡 / 電子線トモグラフイ / オートファジー / タンパク質分解 / 酵母細胞 / 微細構造

Research Abstract

Autophagy is one of the intracellular degradative process. In this process, cells sequester their own cytoplasmic components into a novel double-membrane structure which is newly formed in cytoplasm, and then transport it into the lytic compartment for the reuse. Autophagy defective yeast mutants were isolated by genetic screening. To investigate how these membrane structures are generated during autophagy, I observed intracellular structure of autophagy defective mutants by using an electron microscope. As a result, I found the initial stage of this membrane structure.

Research Products

• [Journal Article] SNARE proteins are required for macroautophagy (2011)
  • Author(s): Nair U, Jotwani A, Geng J, Gammoh N, Richerson D, Yen WL, Griffith J, Nag S, Wang K, Moss T, Baba M, McNew JA, Jiang X, Reggiori F, Melia J, Klionsky DJ
  • Journal Title: Cell Volume: Vol.146 Pages: 290-302
  • URL: http://dx.doi.org/10.1016/j.cell.2011.06.022
  • Peer Reviewed
• [Journal Article] The impact of electron microscopy on autophagy research (2011)
  • Author(s): Esklinen EL, Reggiori F, Baba M, Kovacs AL, Seglen PO
  • Journal Title: Autophagy Volume: Vol.7 Pages: 1-22
  • URL: http://dx.doi.org/10.4161/auto.7.9.15760
  • Peer Reviewed
• [Journal Article] The conserved oligomeric Golgi complex is involved in double-membrane vesicle formation during autophagy (2010)
  • Author(s): Yen WL, Shintani T, Nair U, Cao Y, BaBa M, Klionsky DJ
  • Journal Title: Journal of Cell Biology Volume: Vol.11 Pages: 101-114
  • DOI: doi:10.1083/jcb.200904075
  • Peer Reviewed
• [Journal Article] Double duty of Atg9 self-association in autophagosome biogenesis (2009)
  • Author(s): He C, Baba M, Klionsky DJ
  • Journal Title: Autophagy Volume: Vol.5 Pages: 385-387
  • URL: http://dx.doi.org/10.4161/auto.5.3.7699
  • Peer Reviewed
• [Journal Article] Atg32 is a mitochondrial protein that confers selectivity during mitophagy (2009)
  • Author(s): Kanki T, Wang K, Cao Y, Baba M, Klionsky DJ
  • Journal Title: Developmental Cell Volume: Vol.17 Pages: 98-109
  • DOI: doi:10.1016/j.devcel.2009.06.014
  • Peer Reviewed
• [Journal Article] A genomic screen for yeast mutants defective in selective mitochondria autophagy (2009)
  • Author(s): Kanki T, Wang K, Cao Y, Baba M, Klionsky DJ
  • Journal Title: Molecular Biological of the Cell Volume: Vol.20 Pages: 4730-4738
  • DOI: doi:10.1091/mbc.E09-03-0225
  • Peer Reviewed
• [Presentation] 3D電子線トモグラフィーにおける濃度分布特徴に基づく細胞内構造体の抽出法 (2011)
  • Author(s): 笹島洋輔, 小林博之, 馬場美鈴, 馬場則男
  • Organizer: 第67回日本顕微鏡学会学術講演会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2011-05-16
• [Presentation] 液胞輸送に関与するレセプター蛋白質の挙動の免疫電顕による解析 (2009)
  • Author(s): 馬場美鈴、Jiefei J., Usha N., 馬場則男, Klionsky, D. J.
  • Organizer: 第65回日本顕微鏡学会
  • Place of Presentation: 仙台国際センター
  • Year and Date: 2009-05-29
• [Presentation] 構成画像成分分析に基づく目的像露出法 (2009)
  • Author(s): 唐崎斎、馬場美鈴、馬場則男
  • Organizer: 第65回日本顕微鏡学会
  • Place of Presentation: 仙台国際センター
  • Year and Date: 2009-05-29
• [Presentation] 選択的オートファジーの基質認識機構の形態学的解析 (2009)
  • Author(s): 馬場美鈴
  • Organizer: 第65回日本顕微鏡学会
  • Place of Presentation: 仙台国際センター
  • Year and Date: 2009-05-28