2012 Fiscal Year Final Research Report
Clarification of the physiological role of diacylglycerol in E. coli
| Project/Area Number |
21570110
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Iwate University |
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Principal Investigator |
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| Project Period (FY) |
2009 – 2012
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| Keywords | MPIase / SecG / ジアシルグリセロール / タンパク質膜挿入 / 再構成 / 大腸菌 |
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| Research Abstract |
To clarify the physiological role of diacylglycerol in E. coli, ～10 genes, which may be involved in diacylglycerol biosynthesis, were listed up, and then constructed multiply knock-out strains. Resultantly, an eightfold knock-out strain could be constructed, of which the growth was cold-sensitive and the diacylglycerol content was greatly reduced. However, the depletion was not complete. On the other hand, we determined the structure and analyzed the function of glycolipid MPIase involved in protein integration into membranes.
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[Journal Article] MPIase is a glycolipozyme essential for membrane protein integration2012
Author(s)
Nishiyama, K., Maeda,M., Yanagisawa,K., Nagase, R., Komura, H., Iwashita, T., Yamagaki, T., Kusumoto, S., Tokuda, H., Shimamoto, K.
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Journal Title
Nat. Commun
Volume: 3
Pages: 1-10
DOI
Peer Reviewed
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[Journal Article] A novel complete reconstitution system for membrane integration of the simplest membrane protein, Biochem. Biophys2010
Author(s)
Nishiyama, K., Maeda, M., Abe, M., Kanamori, T., Shimamoto, K., Kusumoto, S., Ueda, T., Tokuda, H.
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Journal Title
Res. Commun
Volume: 394巻
Pages: 733-736
DOI
Peer Reviewed
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