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2011 Fiscal Year Final Research Report

Structural and functional characteristics of natively unfolded protein

Research Project

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Project/Area Number 21570113
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Structural biochemistry
Research InstitutionTottori University

Principal Investigator

KAWATA Yasushi  鳥取大学, 大学院・工学研究科, 教授 (40177697)

Co-Investigator(Renkei-kenkyūsha) MIZOBATA Tomohiro  鳥取大学, 大学院・工学研究科, 准教授 (50263489)
Project Period (FY) 2009 – 2011
Keywordsタンパク質
Research Abstract

In order to understand the relationship between flexibility and function of protein, we investigated intrinsically disordered protein, alpha-synuclein and molecular chaperone, chaperonin in detail from protein chemistry, biochemistry, and biophysical chemistry points of view. We found that the intrinsic flexibility, structural property, and structural changes of polypeptide triggered amyloid fibril formation and also were involved in the function closely.

  • Research Products

    (27 results)

All 2012 2011 2010 2009 Other

All Journal Article (8 results) Presentation (17 results) Remarks (1 results) Patent(Industrial Property Rights) (1 results)

  • [Journal Article] Suppression of Sup35 amyloid fibril formation by group II chaperonin from Thermoplasma Acidophilum2012

    • Author(s)
      Kentaro Noi, Aya Kitamura, Hidenori Hirai, Kunihiro Hongo, Toshihiko Sakurai, Tomohiro Mizobata, and Yasushi Kawata
    • Journal Title

      American J. Mol. Biol

      Volume: (in press)

  • [Journal Article] Varied effects of Pyrococcus furiosus prefoldin and P. furiosus chaperonin on the refolding reactions of substrate proteins2012

    • Author(s)
      Kunihiro Hongo, Hiroshi Itai, Tomohiro Mizobata, and Yasushi Kawata
    • Journal Title

      J. Biochem

      Volume: 151(4) Pages: 383-390

  • [Journal Article] Probing the Functional Mechanism of Escherichia coli GroEL Using Circular Permutation2011

    • Author(s)
      Tomohiro Mizobata, Tatsuya Uemura, Kazuhiro Isaji, Takuma Hirayama, Kunihiro Hongo, and Yasushi Kawata
    • Journal Title

      PLoS ONE

      Volume: 6(10) Pages: e26462

  • [Journal Article] Covalent structural changes in unfolded GroES that lead to amyloid fibril formation detected by NMR : Insight into intrinsically disordered proteins2011

    • Author(s)
      Hisanori Iwasa, Shunsuke Meshitsuka, Kunihiro Hongo, Tomohiro Mizobata, and Yasushi Kawata
    • Journal Title

      J. Biol. Chem

      Volume: 286(24) Pages: 21796-21805

  • [Journal Article] Evaluation of Aβfibrillization inhibitory effect by a PEG-peptide conjugate based on an Aβpeptide fragment with intramolecular FRET2011

    • Author(s)
      Toshihiko Sakurai, Tomoki Iwasaki, Takashi Okuno, Yasushi Kawata, and Naoki Kise
    • Journal Title

      Chem. Commun

      Volume: 47(16) Pages: 4709-4711

  • [Journal Article] Isolation of short peptide fragments from□-synuclein fibril core identifies a residue important for fibril nucleation : A possible implication for diagnostic applications2010

    • Author(s)
      Hisashi Yagi, Hideki Takeuchi, Shiho Ogawa, Naomi Ito, Isao Sakane, Kunihiro Hongo, Tomohiro Mizobata, Yuji Goto, and Yasushi Kawata
    • Journal Title

      Biochim. Biophys. Acta/Proteins and Proteomics

      Volume: 1804(10) Pages: 2077-2087

  • [Journal Article] A potentially versatile nucleotide hydrolysis activity of group II chaperonin monomers from Thermoplasma acidophilum2009

    • Author(s)
      Kentaro Noi, Hidenori Hirai, Kunihiro Hongo, Tomohiro Mizobata, and Yasushi Kawata
    • Journal Title

      Biochemistry

      Volume: 48(40) Pages: 9405-9415

  • [Journal Article] Gly192 at hinge 2 site in the chaperonin GroEL plays a pivotal role in the dynamic apical domain movement that leads to GroES binding and efficient encapsulation of substrate proteins2009

    • Author(s)
      Kodai Machida, Ryoko Fujiwara, Tatsuhide Tanaka, Isao Sakane, Kunihiro Hongo, Tomohiro Mizobata, and Yasushi Kawata
    • Journal Title

      Biochim. Biophys. Acta/Proteins and Proteomics

      Volume: 1794(9) Pages: 1344-1354

  • [Presentation] アントシアニンによる神経変性病原因タンパク質のアミロイド線維形成抑制に関するin vitroとin vivo研究2011

    • Author(s)
      河田康志, 鎌田光博, 渡辺保裕, 小林真菜, 吉田早穂, 田村哲也, 本郷邦広, 溝端知宏, 三浦典正, 中島健二, 小林沙織
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2011-09-24
  • [Presentation] シャペロニンGroELの機能的構造変化の中心点Hinge II部位のGly192残基の変異体機能解析2011

    • Author(s)
      荒木紀帆, 和田翼, 町田広大, 本郷邦広, 溝端知宏, 河田康志
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2011-09-23
  • [Presentation] GroEL円順列変異体CP254の構造・機能相関解析2011

    • Author(s)
      岡田拓也, 植木裕士, 植村達也, 本郷邦広, 河田康志, 溝端知宏
    • Organizer
      第84回日本生化学会大会
    • Place of Presentation
      京都
    • Year and Date
      2011-09-23
  • [Presentation] αシヌクレイン蛋白質のアミロイド線維核部位に位置するPhe 94の線維形成における役割2011

    • Author(s)
      伊藤有未, 武内英樹, 本郷邦広, 溝端知宏, 河田康志
    • Organizer
      第11回日本蛋白質科学会年会
    • Place of Presentation
      吹田
    • Year and Date
      2011-06-09
  • [Presentation] 天然変性蛋白質α-synucleinのアミロイド線維伸張直接観察2011

    • Author(s)
      八木寿梓, 武内英樹, 水野愛子, 河田康志, 後藤祐児
    • Organizer
      第11回日本蛋白質科学会年会
    • Place of Presentation
      吹田
    • Year and Date
      2011-06-08
  • [Presentation] シャペロニン本来の基質蛋白質の同定2011

    • Author(s)
      石橋利嗣, 本郷邦広, 溝端知宏, 河田康志
    • Organizer
      第11回日本蛋白質科学会年会
    • Place of Presentation
      吹田
    • Year and Date
      2011-06-07
  • [Presentation] Role of natively unstructured polypeptide : Molecular chaperone GroEL and alpha-synuclein2011

    • Author(s)
      Yasushi Kawata
    • Organizer
      The 1st International Symposium on Intrinsically Disordered Proteins
    • Place of Presentation
      Yokohama
    • Year and Date
      2011-01-28
  • [Presentation] NMRによるGroESアミロイド線維中間体の形成メカニズムと植物性低分子化合物による阻害効果の評価2010

    • Author(s)
      岩佐尚徳, 吉田早穂, 亀田啓, 飯塚舜介, 本郷邦広, 溝端知宏, 河田康志
    • Organizer
      BMB2010 :第33回日本分子生物学会年会,第83回日本生化学会大会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-09
  • [Presentation] ポリフェノールによる神経変性病原因タンパク質のアミロイド線維の抑制と分解2010

    • Author(s)
      河田康志, 小林真菜, 田村哲也, 岩佐尚徳, 小林沙織
    • Organizer
      BMB2010 :第33回日本分子生物学会年会,第83回日本生化学会大会
    • Place of Presentation
      神戸
    • Year and Date
      2010-12-07
  • [Presentation] Detection of the conformational change by NMR that leads to GroES amyloid fibril formation from a disordered state2010

    • Author(s)
      Hisanori Iwasa, Shunsuke Meshitsuka, Kunihiro Hongo, Tomohiro Mizobata, and Yasushi Kawata
    • Organizer
      The 3^<rd> International Symposium on Protein Community
    • Place of Presentation
      Nara
    • Year and Date
      2010-09-14
  • [Presentation] Isolation of short peptide segments from. lpha-synuclein fibril core identifies a residue important for fibril nucleation2010

    • Author(s)
      Yasushi Kawata, Hisashi Yagi, Hideki Takeuchi, Kunihiro Hongo, Tomohiro Mizobata, and Yuji Goto
    • Organizer
      The 24th Protein Society Meeting
    • Place of Presentation
      San Diego
    • Year and Date
      2010-08-04
  • [Presentation] Rearrangement reaction at Asn-Gly of GroES enhances a conformation that leads to amyloid fibril formation in early step2010

    • Author(s)
      Hisanori Iwasa, Shunsuke Meshitsuka, Kunihiro Hongo, Tomohiro Mizobata, and Yasushi Kawata
    • Organizer
      The 24th Protein Society Meeting
    • Place of Presentation
      San Diego
    • Year and Date
      2010-08-02
  • [Presentation] GroESのアミロイド線維形成の初期過程には核部位直前のアミノ酸配列が関与する2010

    • Author(s)
      岩佐尚徳, 飯塚舜介, 本郷邦広, 溝端知宏, 河田康志
    • Organizer
      第10回日本蛋白質科学会年会
    • Place of Presentation
      札幌
    • Year and Date
      2010-06-16
  • [Presentation] コシャペロニンGroESのアミロイド線維形成と細胞毒性2009

    • Author(s)
      吉田早穂, 岩佐尚徳, 本郷邦広, 溝端知宏, 河田康志
    • Organizer
      第82回日本生化学会大会
    • Place of Presentation
      神戸
    • Year and Date
      2009-10-22
  • [Presentation] Gly192 at hinge 2 site in the chaperonin GroEL plays a pivotal role in the dynamic apical domain movement that leads to GroES binding and efficient encapsulation of substrate proteins2009

    • Author(s)
      Yasushi Kawata, Kodai Machida, Ryoko Fujiwara, Tatsuhide Tanaka, Isao Sakane, Kunihiro Hongo, and Tomohiro Mizobata
    • Organizer
      The 23rd Protein Society Meeting
    • Place of Presentation
      Boston
    • Year and Date
      2009-07-26
  • [Presentation] 天然変性タンパク質のコンフォメーション変化,アミロイド線維形成,細胞毒性(招待講演)2009

    • Author(s)
      河田康志
    • Organizer
      蛋白研究所セミナー「蛋白質立体構造を基盤とするプリオン現象の解明と制御」
    • Place of Presentation
      大阪
    • Year and Date
      2009-07-14
  • [Presentation] 古細菌Thermoplasma acidophilum由来単量体シャペロニンの金属特異的新規機能の解析2009

    • Author(s)
      野井健太郎, 平井秀憲, 本郷邦広, 溝端知宏, 河田康志
    • Organizer
      第50回日本生化学会中国四国支部例会
    • Place of Presentation
      鳥取
    • Year and Date
      2009-05-15
  • [Remarks]

    • URL

      http://www.bio.tottori-u.ac.jp/~prot/main.html

  • [Patent(Industrial Property Rights)] アミロイド線維伸張ペプチド2009

    • Inventor(s)
      河田康志,溝端知宏,本郷邦広,武内英樹
    • Industrial Property Rights Holder
      鳥取大学
    • Industrial Property Number
      特許、特願2009-277681
    • Filing Date
      2009-12-07

URL: 

Published: 2013-07-31  

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