2011 Fiscal Year Final Research Report
Properties of enzymes involved in methionine biosynthesis in mammals
| Project/Area Number |
21570132
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Tokyo University of Agriculture (2011)
Tokyo University of Agriculture and Technology (2009-2010) |
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Principal Investigator |
YAMADA Kazuhiro 東京農業大学, 応用生物科学部, 研究員 (90431973)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 酵素化学 / 栄養化学 |
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| Research Abstract |
Expression and purification of human methionine synthase, methionine synthase reductase(MSR), and methylenetetrahydrofolate reductase(MTHFR) have been achieved. A common polymorphism in MSR gene, 66A> G, did not influence on protein function. Also, effects of gene mutations found in severe MTHFR-deficient patients were examined, i. e. properties of these mutant proteins were biochemically characterized. In addition, protein structural analysis using Thermus thermophilus HB8 MTHFR as model rationally explained how the gene mutations affected protein properties.
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