2011 Fiscal Year Final Research Report
Role of BIG2, a Guanine-Nucleotide Exchanging factor for ADP-Ribosylation Factors, in Insulin-Regulated Glucose Transporter Translocation
Project/Area Number |
21570142
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Functional biochemistry
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Research Institution | The University of Tokushima |
Principal Investigator |
UCHIYAMA Keiji 徳島大学, 疾患酵素学研究センター, 准教授 (60294039)
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Project Period (FY) |
2009 – 2011
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Keywords | Glut4 / BIG2 / Glut4トランスロケーション / インスリン作用 |
Research Abstract |
We here show that BIG2 plays an important role in a formation of Glut4 vesicles from endosomal compartment in both basal and insulin-stimulated adipocytes. In 3T3-L1 adipocytes, knockdown of BIG2 by RNA interference leaded to insufficient insulin-stimulated Glut4 translocation to the plasma membrane. The basal distribution of Glut4 was analyzed by subcellular fractionation. It was shown that the formation of Glut4-vesicles in the BIG2-knockdown cells was strongly repressed. Here, we used an in vitro reconstitution of Glut4-vesicles in order to demonstrate that these Glut4-vesicles are formed from endosomal membranes in a BIG2-dependent manner. Reducing endogenous BIG2 inhibited in vitro formation of Glut4 vesicles from the endosomal compartment prepared from not only unstimulated but insulin-stimulated adipocytes.
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Research Products
(6 results)
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[Journal Article] The Rab GTPase-Activating Protein AS160 as A Common Regulator of Insulin-and G. q-Mediated Intracellular GLUT4 Vesicle Distribution2009
Author(s)
Yuasa, T, Uchiyama K., Ogura, Y., Kimura M., Teshigawara K., Hosaka T., Tanaka, Y., Obata T., Sano H., Kishi K., and Ebina Y.
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Journal Title
Endocrine J.
Volume: Vol.56, No.3
Pages: 345-359
Peer Reviewed
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