2011 Fiscal Year Final Research Report
The study of roles of RACK1 in physiological functions of diacylglycerol kinase(type II)
| Project/Area Number |
21570145
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
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| Project Period (FY) |
2009 – 2011
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| Keywords | シグナル伝達 / 脂質 / 酵素 |
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| Research Abstract |
Knockdown of diacylglycerol kinase_η(DGK_η) using siRNA inhibited cell proliferation of the HeLa cells. Moreover, DGK_ηactivateed the Ras/B-Raf/C-Raf/MEK/ERK signaling pathway induced by epidermal growth factor(EGF). DGK_ηregulated recruitment of B-Raf and C-Raf from cytosol to membranes and their heterodimerization in EGF-stimulated HeLa cells. DGK_ηalso activated C-Raf but not B-Raf in an EGF-dependent manner. These results suggest that DGK_ηacts as a novel critical regulatory component of the ERK signaling pathway. On the other hand, it is suggested that DGKα positively regulates tumor necrosis factor_α-dependent nuclear factor-_κB activation via the protein kinase Cζ-mediated Ser311 phosphorylation of p65 subunit in AKI melanoma cells.
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Research Products
(21 results)
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[Journal Article] Metallothionein deficiency in the injured peripheral nerves of complex regional pain syndrome as revealed by proteomics2012
Author(s)
Oki G, Wada T, Iba K, Aiki H, Sasaki K, Imai S, Sohma H, Matsumoto K, Yamaguchi M, Fujimiya M, Yamashita T and Kokai Y
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Journal Title
Pain
Volume: 153
Pages: 532-539
Peer Reviewed
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[Journal Article] Early expression of plasma CCL8 closely correlates with survival rate of acute graft-vs.-host disease in mice2011
Author(s)
Yamamoto M, Ota A, Hori T, Imai S, Sohma H, Suzuki N, Hatakeyama N, Inazawa N, Ito YM, Kimura H, Tsutsumi H and Kokai Y
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Journal Title
Exp. Hematol
Volume: 39
Pages: 1101-1112
Peer Reviewed
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[Journal Article] Investigation of annexin A5 as a biomarker for Alzheimer's disease using neuronal cell culture and mouse model2010
Author(s)
Yamaguchi M, Kokai Y, Imai S, Utsumi K, Matsumoto K, Honda H, Mizue Y, Momma M, Maeda T, Toyomasu S, Ito YM, Kobayashi S, Hashimoto E, Saito T and Sohma H
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Journal Title
J. Neurosci. Res.
Volume: 88
Pages: 2682-2692
Peer Reviewed
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