Does inhibition of phosphatidylserine synthase induce autophagy?

2011 Fiscal Year Final Research Report

• Project/Area Number: 21570155
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Functional biochemistry
• Research Institution: National Institute of Infectious Diseases
• Principal Investigator: TANIDA Isei 国立感染症研究所, 細胞化学部, 室長 (30296868)
• Project Period (FY): 2009 – 2011
• Keywords: オートファジー / リン脂質代謝 / タンパク質分解 / 細胞死 / ホスファチジルセリ

Research Abstract

To investigate the relation between a defect in phosphatidylserine synthesis and autophagic cell death, we focused on a CHO mutant that has a defect in an activity of phosphatidylserine synthase. When the mutant cells were cultured in a medium lacking phosphatidylserine, LC3-II, a promising autophagic marker, was increased within 24h. At 24-48h after induction of LC3-II, cell viability was decreased. The results suggested that the autophagy play a cytoprotective role, when phsophatidylserine depeletion-induced cell death.

Research Products

• [Journal Article] The FAP motif within human ATG7, an autophagy-related E1-like enzyme, is essential for the E2-substrate reaction of LC3 lipidation (2012)
  • Author(s): Tanida I, Yamasaki M, Komatsu M, Ueno T.
  • Journal Title: Autophagy Volume: 8 Pages: 88-97
  • DOI: DOI:10.4161/auto.8.1.18339
  • Peer Reviewed
• [Journal Article] Doxorubicin-induced glomerulosclerosis with proteinuria in GFP-GABARAP transgenic mice (2012)
  • Author(s): Takagi-Akiba M, Asanuma K, Tanida I, Tada N, Oliva Trejo JA, Nonaka K, Asanuma E, Kominami E, Ueno T, Tomino Y.
  • Journal Title: Am J Physiol Renal Physiol Volume: 302 Pages: F380-9
  • DOI: DOI:10.1152/ajprenal.00502.2010
  • Peer Reviewed
• [Journal Article] Hepatic steatosis inhibits autophagic proteolysis via impairment of autophagosomal acidification and cathepsin expression (2011)
  • Author(s): Inami Y, Yamashina S, Izumi K, Ueno T, Tanida I, Ikejima K, Watanabe S.
  • Journal Title: Biochem Biophys Res Commun Volume: 412 Pages: 618-25
  • DOI: DOI:10.1016/j.bbrc.2011.08.012
  • Peer Reviewed
• [Journal Article] Liver autophagy contributes to the maintenance of blood glucose and amino acid levels (2011)
  • Author(s): Ezaki J, Matsumoto N, Takeda-Ezaki M, Komatsu M, Takahashi K, Hiraoka Y, Taka H, Fujimura T, Takehana K, Yoshida M, Iwata J, Tanida I, Furuya N, Zheng DM, Tada N, Tanaka K, Kominami E, Ueno T.
  • Journal Title: Autophagy Volume: 7 Pages: 727-736
  • DOI: DOI:10.4161/auto.7.7.15371
  • Peer Reviewed
• [Journal Article] Caffeine induces apoptosis by enhancement of autophagy via PI3K/Akt/mTOR/p70S6K inhibition (2011)
  • Author(s): Saiki S, Sasazawa Y, Imamichi Y, Kawajiri S, Fujimaki T, Tanida I, Kobayashi H, Sato F, Sato S, Ishikawa K, Imoto M, Hattori N.
  • Journal Title: Autophagy Volume: 7 Pages: 176-87
  • DOI: DOI:10.4161/auto.7.2.14074
  • Peer Reviewed
• [Journal Article] Autophagy basics (2011)
  • Author(s): Tanida I.
  • Journal Title: Microbiol Immunol Volume: 55 Pages: 1-11
  • DOI: DOI:10.1111/j.1348-0421.2010.00271.x.
  • Peer Reviewed
• [Journal Article] Autophagosome Formation and Molecular Mechanism of Autophagy (2011)
  • Author(s): Tanida I.
  • Journal Title: Antioxid Redox Signal Volume: 14 Pages: 2201-14
  • DOI: DOI:10.1089/ars.2010.3482
  • Peer Reviewed
• [Journal Article] オートファジー-リソソーム分解系～疾患・生理機能との関わりと分子機構 (2011)
  • Author(s): 谷田以誠
  • Journal Title: 内分泌・糖尿病・代謝内科、和文総説 Volume: 33 Pages: 319-325
  • URL: http://www.kahyo.com/item/B201110-334
• [Journal Article] オートファジーの評価方法、実験医学 (2011)
  • Author(s): 谷田以誠、上野隆、和栗聡
  • Journal Title: 和文総説 Volume: 29 Pages: 2054-2062
  • URL: http://www.yodosha.co.jp/bookdata/9784758103169/9784758103169_contents.pdf
• [Journal Article] Measurement of autophagy in cells and tissues (2010)
  • Author(s): Tanida I, Waguri S.
  • Journal Title: Methods Mol Biol Volume: 648 Pages: 193-214
  • DOI: DOI:10.1007/978-1-60761-756-3_13
  • Peer Reviewed
• [Journal Article] Autophagic compartments gain access to the MHC class II compartments in thymic epithelium (2009)
  • Author(s): Kasai M, Tanida I, Ueno T, Kominami E, Seki S, Ikeda T, Mizuochi T.
  • Journal Title: J Immunol Volume: 183 Pages: 7278-7285
  • URL: http://www.jimmunol.org/content/183/11/7278.long
  • Peer Reviewed
• [Journal Article] Knockdown of autophagy-related gene decreases the production of infectious hepatitis C virus particles (2009)
  • Author(s): Tanida I, Fukasawa M, Ueno T, Kominami E, Wakita T, Hanada K.
  • Journal Title: Autophagy Volume: 5 Pages: 937-945
  • URL: http://www.landesbioscience.com/journals/autophagy/article/9243/?nocache=162694941
  • Peer Reviewed
• [Journal Article] Inhibition of hepatitis C virus replication by chloroquine targeting virus-associated autophagy (2009)
  • Author(s): Mizui T, Yamashina S, Tanida I, Takei Y, Ueno T, Sakamoto N, Ikejima K, Kitamura T, Enomoto N, Sakai T, Kominami E, Watanabe S.
  • Journal Title: J Gastroenterol Volume: 45 Pages: 195-203
  • DOI: DOI:10.1007/s00535-009-0132-9
  • Peer Reviewed
• [Presentation] Ubiquitin modifies an autophagic modifier to inhibit ALIPS formation (2012)
  • Author(s): Tanida, I., Koike, M., Ueno, T., Uchiyama, Y.
  • Organizer: Gordon Research Conferences "Autophay in Stress, Development and Disease"
  • Place of Presentation: Ventura, CA, USA
  • Year and Date: 20120311-17
• [Presentation] 新奇構造体ALIPS形成抑制のためのユビキチン・プロテアソームによるATG12分解制御 (2011)
  • Author(s): 谷田以誠、小池正人、上野隆、内山安男
  • Organizer: 第34回日本分子生物学会年
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-15
• [Presentation] Stability of luciferase activity during autophagy (2010)
  • Author(s): Tanida, I., Hanada, K.
  • Organizer: 50th Annual Meeting of American Society of Cell Biology
  • Place of Presentation: Philadelphia, PA, U. S. A.
  • Year and Date: 20101211-17
• [Presentation] オートファジー条件におけるプロテアソームの活性化 (2010)
  • Author(s): 谷田以誠、花田賢太郎
  • Organizer: BMB2010
  • Place of Presentation: 神戸
  • Year and Date: 20101207-10
• [Presentation] Autophagic Mechanism and Production of HCV Particles (2010)
  • Author(s): Tanida, I.
  • Organizer: World Congress of Virus and Infection-2010
  • Place of Presentation: Busan, Korea
  • Year and Date: 20100730-0804
• [Presentation] Autophagy and mTor-signaling in production of infectious HCV-particles (2010)
  • Author(s): Tanida, I., Fukasawa, M., Ueno, T., Kominami, E., Wakita, T., Hanada, K.
  • Organizer: Gordon Research Conferences "Autophay in Stress, Development and Disease", Il Ciocco
  • Place of Presentation: Barga, Italy
  • Year and Date: 20100425-30
• [Presentation] Visualization of GABARAP in mice (2009)
  • Author(s): Tanida, I., Koike, M., Tada, N., Iwata, J., Ueno, T., Uchiyama, Y., Kominami, E., Hanada, K.
  • Organizer: 49th Annual Meeting of American Society of Cell Biology
  • Place of Presentation: San Diego, U. S. A.
  • Year and Date: 2009-12-08
• [Presentation] オートファジーはHCV粒子産生に関与している (2009)
  • Author(s): 谷田以誠、深澤征義、脇田隆字、花田賢太郎
  • Organizer: 第57回日本ウィルス学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2009-10-25
• [Presentation] オートファジー関連遺伝子のノックダウンはHCV粒子産生を抑制する (2009)
  • Author(s): 谷田以誠、深澤征義、脇田隆字、上野隆、木南英紀、花田賢太郎
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-23
• [Presentation] Autophagy in Naive Hepatitis C Virus Production (2009)
  • Author(s): Tanida, I.
  • Organizer: 5th International Symposium on Autophagy
  • Place of Presentation: Shiga, Japan
  • Year and Date: 2009-09-27
• [Presentation] Essential role of liver autophagy in maintaining blood glucose and plasma amino acids (2009)
  • Author(s): Ueno, T., Ezaki, J., Matsumoto, N., Takeda-Ezaki, M., Komatsu, M., Fujimura, T., Tanida, I., Furuya, N., Kominami, E.
  • Organizer: 5th International Symposium on Autophagy
  • Place of Presentation: Shiga, Japan
  • Year and Date: 2009-09-27
• [Presentation] Autophagy Basics (2009)
  • Author(s): Tanida, I.
  • Organizer: International Symposium on Autophagy and Cell Death
  • Place of Presentation: Tokyo, Japan
  • Year and Date: 2009-07-06
• [Remarks]
  • URL: http://www.nih.go.jp/niid/ja/from-biochem/931-2nd.html