2011 Fiscal Year Final Research Report
Characterization of enzymatic property plant specific class VIII myosin
| Project/Area Number |
21570159
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Chiba University |
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Principal Investigator |
ITO Kohji 千葉大学, 大学院・理学研究院, 准教授 (50302526)
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| Project Period (FY) |
2009 – 2011
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| Keywords | ミオシン / 分子モーター / アクチン / シロイヌナズナ / 植物 / キネティクス / 遊離マグネシウムイオン |
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| Research Abstract |
Myosin is motor protein which converts chemical energy into mechanical movement. Phylogenetic analysis revealed that myosins consist of at least 35 classes. Among these classes, plant has only 2 classes, namely, class VIII and class XI. although enzymatic and motile properties of Arabidopsis class VIII myosin, ATM. We have found that actin sliding velocity by ATm was very slow and actin-activated ATPase activity of ATm was very low. In addition, we also found that both the actin sliding velocity and the actin-activated ATPase activities were regulated by free Mg^<2+>. Kinetic analyses using stopped flow apparatus revealed that free Mg^<2+>controled ADP dissociation rate from acto-myosin-ADP.
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