2011 Fiscal Year Final Research Report
Construction of an atomic model of the skeletal muscle thin filament by FRET measurements
| Project/Area Number |
21570162
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | University of Fukui |
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Principal Investigator |
MIKI Masao 福井大学, 大学院・工学研究科, 教授 (30242580)
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| Project Period (FY) |
2009 – 2011
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| Keywords | タンパク質 / 核酸の構造 / 動態 / 機能 |
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| Research Abstract |
Striated muscle thin filament is composed of actin, tropomyosin(Tm), and troponin(Tn). The fluorescence resonance energy(FRET) donor probe was labeled at the specific sites on tropomyosin or troponin, and the energy acceptor probes were labeled at the specific sites on actin. From these donor and acceptor pairs, FRET efficiencies were determined. Using the atomic coordinates of F-actin, Tm, and Tn, we searched all possible arrangements for Tn and Tm on F-actin to calculate FRET efficiency for each donor-acceptor pair in each arrangement. By minimizing the squared sum of deviations for the calculated FRET efficiencies from the observed efficiencies, we determined the location of Tm and Tn on F-actin.
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Research Products
(11 results)
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[Journal Article] A Three-Dimensional FRET Analysis to Construct an Atomic Model of the Actin. Tropomyosin. Troponin Core Domain Complex on a Muscle Thin Filament2012
Author(s)
Miki, M., Makimura, S., Sugahara, Y., Yamada, R., Bunya, M., Saitoh, T., & Tobita, H.
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Journal Title
J. Mol. Biol
Volume: 420
Pages: 40-55
Peer Reviewed
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