2011 Fiscal Year Final Research Report
Development of controlling technology for protein dissociation and aggregation using high pressure.
| Project/Area Number |
21570163
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Gifu University |
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Principal Investigator |
KAMATARI Yujio 岐阜大学, 生命科学総合研究支援センター, 助教 (70342772)
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| Co-Investigator(Kenkyū-buntansha) |
MUTO Junji 科学警察研究所, 法科学第一部, 主任研究官 (80432186)
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| Co-Investigator(Renkei-kenkyūsha) |
YAMAGUCHI Keiichi 岐阜大学, 連合創薬医療情報研究科, 特別研究員(PD)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 生物物理学 / フォールディング / 神経変性疾患 / プリオン病 / 凝集 / 圧力 / 巻き戻り / リゾチーム |
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| Research Abstract |
Protein aggregation is one of the serious problems for human health and production of recombinant proteins. Purpose of this study is development of controlling technology for protein dissociation and aggregation using high pressure. In this study, we did following four topics. 1. We observed denaturation process of hen lysozyme using high pressure NMR spectroscopy, in which high pressure suppress protein aggregation. We succeed to observe low-lying excited states and unfolding intermediates at equilibrium condition(Kamatari et al., Biophys Chem. 156, 24-30, 2011). 2. We made a high-volume high pressure apparatus and did refolding experiments from inclusion bodies. 3. We made a high pressure apparatus for fluorescence spectroscopy and observed dissociation process of amyloid fibrils. 4. We used chemical compounds to suppress aggregation in addition to high pressure. We found several new anti-prion compounds(Hosokawa-Muto et al., Antimicrob. Agents Chemother., 53, 765-771, 2009). We optimized a anti-prion compound, GN8, and found higher efficiency compound than GN8(Kimura et al., Bioorg Med Chem Lett. 21, 1502-1507, 2011).
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[Journal Article] 3, 4-Dicaffeoylquinic Acid, a Major Constituent of Brazilian Propolis, Increases TRAIL Expression and Extends the Lifetimes of Mice Infected with the Influenza A Virus.2011
Author(s)
Takemura T, Urushisaki T, Fukuoka M, Hosokawa-Muto J, Hata T, Okuda Y, Hori S, Tazawa S, Araki Y, Kuwata K.
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Journal Title
Evid. Based Complement. Alternat. Med.
Peer Reviewed
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