2011 Fiscal Year Final Research Report
Analysis of physicochemical properties and functions of quinone molecule in the homodimeric photosynthetic reaction center
| Project/Area Number |
21570168
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | Osaka University |
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Principal Investigator |
OH-OKA Hirozo 大阪大学, 大学院・理学研究科, 准教授 (30201966)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 光合成 / 反応中心 / 電子移動 / 形質転換 / 緑色硫黄細菌 |
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| Research Abstract |
The novel molecular biological system, which artificially alter the homodimeric structure of the photosynthetic reaction center(RC) to the heterodimeric one, has been constructed in the green sulfur bacterium, Chlorobaculum tepidum. By the tandem chromatographic procedures using Ni-NTA and Strep-tactin resins, the Strep-PscA/ His-PscA-type RC could be obtained successfully. The method for the transformation system with a broad-host-range plasmid has also been developed to simplify genetic analyses, and will be a useful tool in the near future.
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[Journal Article] A novel monogalactosyldiacylglycerol synthase found in green sulfurbacteria reveals new roles for galactolipids in photosynthesis.2011
Author(s)
S. Masuda, J. Harada, M. Yokono, Y. Yuzawa, M. Shimojima, K. Murofushi, H. Tanaka, H. Masuda, M. Murakawa, T. Haraguchi, M. Kondo, M. Nishimura, H. Yuasa, M. Noguchi, H. Oh-oka, A. Tanaka, H. Tamiaki and H. Ohta
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Journal Title
Plant Cell
Volume: 23
Pages: 2644-2658
Peer Reviewed
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[Journal Article] An overview on chlorophylls and quinones in the photosystem I-type reaction centers.2010
Author(s)
S. Ohashi, T. Iemura, N. Okada, S. Itoh, H. Furukawa, M. Okuda, M. Ohnishi-Kameyama, T. Ogawa, H. Miyashita, T. Watanabe, S. Itoh, H. Oh-oka, K. Inoue and M. Kobayashi
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Journal Title
Photosynth. Res.
Volume: 104
Pages: 305-319
Peer Reviewed
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[Presentation] Identification of a novel monogalactosyldiacylglycerol synthase from the photosynthetic green sulfur bacterium Chlorobium tepidum2009
Author(s)
S. Masuda, J. Harada, Yokono, M. Shimojima, K. Murofushi, Y. Yuzawa, M. Murakawa, H. Oh-oka, A. Tanaka, H. Tamiaki, and H. Ohta
Organizer
3rd Asian Symposium on Plant Lipids
Place of Presentation
Yokohama, Japan
Year and Date
20091127-29
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