2011 Fiscal Year Final Research Report
Resonance Raman Study on the Proton Pumping Mechanism of Cytochrome c Oxidase
| Project/Area Number |
21570171
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Biophysics
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| Research Institution | University of Hyogo |
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Principal Investigator |
OGURA Takashi 兵庫県立大学, 大学院・生命理学研究科, 教授 (70183770)
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| Research Collaborator |
YANAGISAWA Sachiko 兵庫県立大学, 大学院・生命理学研究科, 助教 (60557982)
NAKASHIMA Satoru 兵庫県立大学, 大学院・生命理学研究科, 特任准教授 (80263234)
KUBO Minoru 兵庫県立大学, 大学院・生命理学研究科, 特任助教 (90392878)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 好気呼吸 / 生体エネルギー変換 / チトクロムC酸化酵素 / 酸素還元 / プロトンポンプ / 共鳴ラマン / 部位特異的アミノ酸置換 |
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| Research Abstract |
Cytochrome c oxidase plays an essentially important function in cell respiration to maintain life, and catalyzes dioxygen reduction to water, which is coupled with proton pumping across the membrane. In order to elucidate the mechanism of this enzyme, high resolution structures at a picometer level are to be determined by resonance Raman spectroscopy. In the present study, we have established assignments of some Raman siganals. In addition, an intermediate conformational state and cooperative dynamics of the two hemes have been detected, which are essential for an efficient proton pump.
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[Journal Article] Post-translational His-Cys Cross Linkage Formation in Tyrosinase Induced by Copper(II)-Peroxo Species2011
Author(s)
Nobutaka Fujieda, Takuya Ikeda, Michiaki Murata, Sachiko Yanagisawa, Shigetoshi Aono, Kei Okubo, Satoshi Nagao, Takashi Ogura, Shun Hirota, Shunichi Fukuzumi, Yukihiro Nakamura, Yoji Hata, and Shinobu Itoh
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Journal Title
J. Am. Chem. Soc.
Volume: 133
Pages: 1180-1183
DOI
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