Regulatory mechanism for intracellular transport depending on the DLK-JNK signaling pathway

2012 Fiscal Year Final Research Report

• Project/Area Number: 21570184
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Molecular biology
• Research Institution: Wakayama Medical University (2012); Yokohama City University (2009-2011)
• Principal Investigator: HIRAI Syu-ichi 和歌山県立医科大学, 医学部, 教授 (80228759)
• Project Period (FY): 2009 – 2012
• Keywords: JNK / JIP / Kinesin-1 / 細胞内輸送 / 微小管 / 神経軸索

Research Abstract

Intracellular trafficking is one of crucial factors supporting cell differentiation and function. This depends on motor proteins, which carry different kinds of cargos, and cytoskeletons, which serve as scaffold or rail of motor proteins. We showed that a protein kinase DLK affect the intracellular trafficking via two different pathways. One is the regulation of microtubules via the activation of JNK, and the other is the regulation of the binding between kinesin motor and cargo molecules via adaptor protein JIP. These findings give new insight into the molecular mechanism regulating intracellular trafficking.

Research Products

• [Journal Article] the interaction of Kinesin-1 with its adaptor protein JIP1 can be regulated via proteins binding to the JIP1-PTB domain (2013)
  • Author(s): T.Satake,K.Ohtsuki,Y.Banba,J.Suenaga,H.Hirano,Y.Yamanaka,S.Ohno,S.Hirai
  • Journal Title: BMC Cell Biol Volume: 14 Pages: 12
  • Peer Reviewed
• [Journal Article] Functional genomic screening identifies dual leucine zipper kinase as a key (2013)
  • Author(s): D.S.Welsbie,Z.Yang,Y.Ge,K.L.Mitchell,X.Zhou,S.E.Martin,C.A.Berlinicke,L.HacklerJr,J.Fuller,J.Fu,L.H.Cao,B.Han,D.Auld,T.Xue,S.Hirai,L.Germain,C.SimardBisson,R.Blouin,J.V.Nguyen,C.H.Davis,R.A.Enke,S.L.Boye,S.L.Merbs,N.Marsh-Armstrong,W.W.Hauswirth,A.Diantonio,R.W.Nickells,J.Inglese,J.Hanes,K.W.Yau,H.A.Quigley,D.J.Zack
  • Journal Title: mediator of retinal ganglion cell death Volume: 110 Pages: 4045-4050
  • Peer Reviewed
• [Journal Article] 軸索形成に必要なDLK-JNK経路と微小管制御 (2012)
  • Author(s): 平井秀一
  • Journal Title: 生体の科学 Volume: 63 Pages: 183-188
  • Peer Reviewed
• [Journal Article] Axon formation in neocortical neurons depends on stage-specific regulation of microtubule stability by the Dual Leucine zipper Kinase-c-Jun N-terminal Kinase pathway (2011)
  • Author(s): S.Hirai,Y.Banba,T.Satake,S.Ohno
  • Journal Title: J.Neurosci Volume: 31 Pages: 6468-6480
  • Peer Reviewed
• [Journal Article] Maintenance of dendritic spine morphology by partitioning-defective 1b through regulation of microtubule growth (2011)
  • Author(s): K.Hayashi,A.Suzuki,S.Hirai,Y.Kurihara,C.C.Hoogenraad,S.
  • Journal Title: J。Neurosci Volume: 31 Pages: 12094-12103
  • Peer Reviewed
• [Journal Article] JNK phosphorylates Ser332 of doublecortin and regulates its function in neurite extension and neuronal migration (2010)
  • Author(s): J.Jin,H.Suzuki,S.Hirai,K.Mikoshiba,T.Ohshima
  • Journal Title: Dev.Neurobiol Volume: 70 Pages: 929-942
  • Peer Reviewed
• [Journal Article] Dominant-negative mutations in a-II spectrin cause West syndrome with severe cerebral hypomyelination (2010)
  • Author(s): H.Saitsu,J.Tohyama,T.Kumada,K.Egawa,K.Hamada,I.Okada,T.Mizuguchi,H.Osaka,shR.Miyata,T.Furukawa,K.Haginoya,H.Hoshino,T.Goto,Y.Hachiya,T.Yamagata,S.Saitoh,T.Nagai,K.Niiyama,A.Nishimura,N.Miyake.M.Komada,K.Hayashi,S.Hirai,K.Ogata,M.Kato,A.Fukuda,N.Matsumoto
  • Journal Title: spastic quadriplegia, and developmental delay Volume: 86 Pages: 1-11
  • Peer Reviewed
• [Journal Article] がん細胞を知る-分子レベルでみた正常細胞との違い- (2010)
  • Author(s): 平井秀一
  • Journal Title: Medical Technology Volume: 38 Pages: 503-506
• [Presentation] JNK reduces SCG10 protein level to regulate axon formation (2012)
  • Author(s): Hirai S,Ohno
  • Organizer: 2012 Annual Meeting of The American Society for Cell Biology
  • Place of Presentation: San Francisco, USA
  • Year and Date: 2012-12-19
• [Presentation] JNK facilitates the formation of epithelial cell sheet (2012)
  • Author(s): Furukawa K, Ohno S, Hirai S
  • Organizer: 第33回日本分子生物学会年会、第83回日本生化学会合同大会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-11
• [Presentation] Molecular mechanism supporting transitions in cell polarity during neuronal development (2011)
  • Author(s): Hirai S
  • Organizer: The 34thAnnual Meeting of the Molecular Biology Society of Japan
  • Place of Presentation: Yokohama
  • Year and Date: 2011-12-15
• [Presentation] Axon formation in neocortical neurons depends on the stage-specific stabilization of microtubules by DLK-JNK pathway (2010)
  • Author(s): Hirai S, Banba Y, Satake T, Ohno S
  • Organizer: 第33回日本分子生物学会年会,第83回日本生化学会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08
• [Presentation] egulatory mechanism for Kinesin-1 motor activity through JIP1 (2010)
  • Author(s): Satake T, Banba Y, Ohno S, Hirai S
  • Organizer: 第33回日本分子生物学会年会、第83回日本生化学会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08
• [Presentation] egulatory mechanism for Kinesin-1 motor activity through JIP1 (2009)
  • Author(s): Satake T, Banba Y, Ohno S, Hirai S
  • Organizer: 第31回日本分子生物学会年会、第81回日本生化学会合同大会
  • Place of Presentation: 横浜
  • Year and Date: 2009-12-12
• [Remarks]
  • URL: http://www.wakayama-med.ac.jp/med/lasbi
• [Remarks]
  • URL: http://ology1/Biology_Index.html