Biological role of condensin in chromosome condensation

2011 Fiscal Year Final Research Report

• Project/Area Number: 21570188
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Molecular biology
• Research Institution: National Institute for Basic Biology
• Principal Investigator: JOHZUKA Katsuki 基礎生物学研究所, 多様性生物学研究室, 助教 (40291893)
• Project Period (FY): 2009 – 2011
• Keywords: 染色体構造 / クロマチン / 組換え / 転写 / サイレンシング

Research Abstract

Chromosome condensation is a basic cellular process that ensure the faithful segregation of chromosomes during cell division. This process is required not only for shrinking the length of chromosome arms, but also for resolving entanglements between sister-chromatids that created during DNA replication. In addition, chromosome condensation plays an important role in maintenance of the stability of repetitive structure in genome such as rDNA repeat. Studies in the past decade have demonstrated that chromosome condensation is mainly achieved by condensin, a multi-subunit protein complex widely conserved from yeast to human. In this study, we showed that condensin contributes for transcriptional-and recombinational-repressions within the rDNA repeat in Saccharomyces cerevisiae.

Research Products

• [Journal Article] The cis element and factors required for condensing recruitment to chromosomes (2009)
  • Author(s): Johzuka K., Horiuchi T.
  • Journal Title: Mol Cell Volume: 34 Pages: 26-35
  • Peer Reviewed
• [Presentation] コンデンシンによるクロマチン折りたたみ (2012)
  • Author(s): 定塚勝樹
  • Organizer: 第29回染色体ワークショップ
  • Place of Presentation: 仙台市秋保温泉
  • Year and Date: 2012-01-27
• [Presentation] Condensin-dependent chromatin folding (2011)
  • Author(s): Johzuka K.
  • Organizer: 第34回日本分子生物学会
  • Place of Presentation: 横浜市パシフィコ横浜
  • Year and Date: 2011-12-15
• [Presentation] コンデンシンによる染色体凝縮の分子機構 (2011)
  • Author(s): 定塚勝樹
  • Organizer: DNA複製組換ワークショップ
  • Place of Presentation: 福岡市サンピア福岡
  • Year and Date: 2011-10-27
• [Presentation] Association of condensin with chromosome : mechanism and its effect on transcriptional silencing (2010)
  • Author(s): Johzuka K.
  • Organizer: 57th NIBB Conference
  • Place of Presentation: 岡崎市岡崎カンファレンスセンター
  • Year and Date: 20101014-16
• [Presentation] コンデンシンによる転写と組換え抑制 (2010)
  • Author(s): 定塚勝樹
  • Organizer: BMB2010シンポジウム
  • Place of Presentation: 神戸市神戸国際展示場
  • Year and Date: 2010-12-10
• [Presentation] Condensin-dependent transcriptional silencing (2010)
  • Author(s): Itazu M., Johzuka K.
  • Organizer: International 3R Symposium
  • Place of Presentation: 富山市富山国際会議場
  • Year and Date: 2010-10-26
• [Presentation] Condensin contributes to the transcriptional silencing in the rDNA cluster (2009)
  • Author(s): Itazu M., Johzuka K.
  • Organizer: 日本分子生物学会
  • Place of Presentation: 横浜市パシフィコ横浜
  • Year and Date: 20091209-12
• [Presentation] コンデンシンによるrDNAリピートでの転写抑制機構 (2009)
  • Author(s): 板津昌子、定塚勝樹
  • Organizer: 第20回DNA複製・組換え・修復ワークショップ
  • Place of Presentation: 彦根市琵琶湖コンファレンスセンター
  • Year and Date: 20091101-03
• [Presentation] コンデンシンによる転写抑制 (2009)
  • Author(s): 定塚勝樹
  • Organizer: 日本遺伝学会
  • Place of Presentation: 松本市信州大学
  • Year and Date: 20090916-18
• [Presentation] 出芽酵母セントロメア近傍での転写抑制 (2009)
  • Author(s): 定塚勝樹、板津昌子、石根直美
  • Organizer: 酵母遺伝学フォーラム
  • Place of Presentation: 筑波市ノバホール
  • Year and Date: 20090728-30
• [Remarks] (1)ホームページ等
  • URL: http://www.nibb.ac.jp/sections/evolutionary_biology_and_biodiversity/diversity/AssisProf/johzuka.html
• [Remarks] (2)報道関連情報(1)「たんぱく質と結合」日経産業新聞2009年4月10日11面
• [Remarks] (2)「結合メカニズム解明」日刊工業新聞2009年4月16日23面
• [Remarks] (3)「染色体凝縮の謎にメス」科学新聞2009年4月24日4面