2011 Fiscal Year Final Research Report
Analysis for the two distinct pathways to form mitotic spindles in C. elegans embryos
| Project/Area Number |
21570209
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
TOYA Mika 独立行政法人理化学研究所, 高次構造形成研究グループ, 研究員 (80455360)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 紡錘体形成 / 微小管制御 / オーロラキナーゼA / γチューブリン / 線虫初期胚 / ライブイメージング / RNAi |
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| Research Abstract |
The evolutionarily conservedγ-tubulin is the main microtubule nucleator. C. elegans embryos have another,γ-tubulin-independent, microtubule assembly mechanism that requires Aurora A kinase(AIR-1). This study showed that AIR-1 stabilizes the spindle microtubules in a kinase-independent manner in addition to its kinase-dependent role at centrosomes. The kinase-independent AIR-1 was crucial in the assembly of chromatin-stimulated microtubules whileγ-tubulin complex was dispensable for the process. The results obtained suggested that the roles of aγ-tubulin complex, a kinase-dependent AIR-1, and a kinase-independent AIR-1 necessary to be coordinated to assemble functional mitotic spindles.
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[Journal Article] Caenorhabditis elegans ortholog of the p24/p22 subunit, DNC-3, is essential for the formation of the dynactin complex by bridging DNC-1/p150(Glued) and DNC-2/dynamitin2010
Author(s)
Terasawa, M., Toya, M., Motegi, F., Mana, M., Nakamura, K. and Sugimoto, A.
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Journal Title
Genes Cells
Volume: 15
Pages: 1145-1157
Peer Reviewed
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