Bidirectional evolution of an ancestral gene for sex determination : testis and ovary determination

2011 Fiscal Year Final Research Report

• Project/Area Number: 21570239
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Evolutionary biology
• Research Institution: Hiroshima University
• Principal Investigator: MIURA Ikuo 広島大学, 大学院・理学研究科, 准教授 (10173973)
• Co-Investigator(Renkei-kenkyūsha): OHTANI Hiromi 広島大学, 大学院・理学研究科, 助教 (20106800) ; ICHIKAWA Youko 県立広島大学, 生命環境学部, 准教授 (20084163)
• Project Period (FY): 2009 – 2011
• Keywords: 性決定 / SRY / 卵巣決定 / SOX3

Research Abstract

We examined the function of the sex-linked SOX3 for ovarian differentiation in the frog Rana rugosa. SOX3 is known to be an ancestral gene of the male determining gene SRY in eutherian mammals. Short nucleotide elements specific to the upstream regions of W-SOX3 and Z-SOX3, respectively, were identified. In the undifferentiated gonads of ZW, W-SOX3 was highly expressed and the higher expression preceded the onset of sexually dimorphic expression of CYP19 and FOXL2. Using transgenesis, we successfully obtained ZZ frogs of which germ cells were introduced by the W-SOX3, but their gonads did not differentiate ovaries.

Research Products

• [Journal Article] Independent degeneration of the W and Ysex chromosomes in frog Rana rugosa (2012)
  • Author(s): Miura I, Ohtani H, and Ogata M
  • Journal Title: Chromosome Res. Volume: 20 Pages: 47-55
  • DOI: DOI:10.1007/s10577-011-9258-8
  • Peer Reviewed
• [Journal Article] The postzygoitc isolation of a unique morphotype of frog Rana rugosa found on Sado Island (2012)
  • Author(s): Ohtani H, Sekiya K, Ogata M, and Miura I
  • Journal Title: Japan. J. Herpet. F Volume: (In press)
  • Peer Reviewed
• [Journal Article] An X-linked body color gene of the frog Rana rugosa and its application to the molecular analysis of gonadal sex differentiation (2011)
  • Author(s): Miura I, Kitamoto H, Koizumi Y, Ogata M, and Sasaki K.
  • Journal Title: Sex Develop Volume: 5 Pages: 250-258
  • DOI: DOI:10.1159/000330365
  • Peer Reviewed
• [Journal Article] Evidence for true fall-mating in Japanese newt Cynops pyrrhogaste (2011)
  • Author(s): Akiyama S, Iwao Y, and Miura I
  • Journal Title: Zoolgical Science Volume: 28 Pages: 758-763
  • URL: http://dx.doi.org/10.2108/zsj.28.758
  • Peer Reviewed
• [Journal Article] ツチカ-エルの日本国内における生息場所について (2011)
  • Author(s): 尾形光昭、三浦郁夫
  • Journal Title: 爬虫両棲類学報 Volume: 2011(1) Pages: 24-25
  • Peer Reviewed
• [Journal Article] Phyletic diversity in the frog Rana rugosa(Anura : Ranidae) with special reference to aunique morphotype found from Sado Island (2010)
  • Author(s): Sekiya K, Ohtani H, Ogata, M, and Miura I
  • Journal Title: Japan. Current Herpetology Volume: 29(2) Pages: 69-78
  • URL: http://dx.doi.org/10.3105/018.029.0202
  • Peer Reviewed
• [Journal Article] カエルにおける色彩発現の遺伝的メカニズム (2009)
  • Author(s): 三浦郁夫
  • Journal Title: 爬虫両棲類学会報 Volume: 2009(2) Pages: 151-160
  • Peer Reviewed
• [Journal Article] 2つの性決定機構をもつツチガエル (2009)
  • Author(s): 尾形光昭、三浦郁夫
  • Journal Title: 生物の科学遺伝 Volume: 1 Pages: 32-37
  • Peer Reviewed
• [Presentation] Recent topics on sex of the frogRana rugosa (2011)
  • Author(s): Miura I
  • Organizer: Workshop Evolution of sexchromosomes and sex determination invertebrates
  • Place of Presentation: Canberra, Australia
  • Year and Date: 20110400
• [Presentation] 生殖腺の性分化決定機構の進化?性ホルモン依存から非依存へ? (2011)
  • Author(s): 三浦郁夫、小泉雄紀, 大谷浩己、尾形光昭
  • Organizer: 染色体学会
  • Place of Presentation: 平塚市
  • Year and Date: 2011-11-11
• [Presentation] ナガレタゴガエルの性染色体 (2011)
  • Author(s): 三浦郁夫、綿貫岳人、市川洋子、藤田宏之
  • Organizer: 日本爬虫両生類学会
  • Place of Presentation: 京都市
  • Year and Date: 2011-10-10
• [Presentation] 佐渡島産ツチガエルの鳴き声について (2011)
  • Author(s): 尾形光昭、関谷國男、長谷川嘉則、大谷浩己、三浦郁夫
  • Organizer: 日本爬虫両生類学会
  • Place of Presentation: 京都市
  • Year and Date: 2011-10-10
• [Presentation] ナゴヤダルマガエルの遺伝的地域分化(II) (2011)
  • Author(s): 長井悠佳里、土井敏男、伊藤邦夫、藤谷武史、湯浅義明、小泉雄紀, 尾形光昭、三浦郁夫
  • Organizer: とくに岡山集団と名古屋集団が接する境界領域について―日本爬虫両生類学会
  • Place of Presentation: 京都市
  • Year and Date: 2011-10-10
• [Presentation] 脊椎動物の体色変異体を用いた (2011)
  • Author(s): 原とおる、山本博章、三浦郁夫、古賀章彦
  • Organizer: 日本遺伝学会
  • Place of Presentation: 京都市
  • Year and Date: 2011-09-21
• [Presentation] ツチガエルの地域集団における生殖腺性差構築機構の解明文部科学省:新学術領域研究性差構築の分子基盤 (2010)
  • Author(s): 三浦郁夫
  • Organizer: 第2回領域会議・公開研究会
  • Place of Presentation: 逗子(神奈川)
  • Year and Date: 20101215-17
• [Presentation] XY型とZW型の遺伝子発現のちがい (2010)
  • Author(s): 小泉雄紀、大谷浩己、三浦郁夫
  • Organizer: 染色体学会第61回年会
  • Place of Presentation: 習志野(千葉)
  • Year and Date: 20101105-07
• [Presentation] ナゴヤダルマガエルの遺伝的地域分化 (2010)
  • Author(s): 長井悠佳里、土井敏男、湯浅義明、藤谷武史、伊藤邦夫、小泉雄紀、三浦郁夫
  • Organizer: 日本爬虫両生類学会第49回大会
  • Place of Presentation: 横浜(神奈川)
  • Year and Date: 20101009-10
• [Presentation] ニホンアカガエルの新規色彩変異-体色と眼は正常だがメスのもつ卵だけが白い (2010)
  • Author(s): 小泉雄紀、森淳、三浦郁夫
  • Organizer: 日本爬虫両生類学会第49回大会
  • Place of Presentation: 横浜(神奈川)
  • Year and Date: 20101009-10
• [Presentation] 佐渡島産ツチガエルの外部形態について (2010)
  • Author(s): 尾形光昭、関谷國男、三浦郁夫
  • Organizer: 日本爬虫両生類学会第49回大会
  • Place of Presentation: 横浜(神奈川)
  • Year and Date: 20101009-10
• [Presentation] オタマジャクシの性を色で見分ける?山口県産ツチガエルの性に連鎖した色彩変異遺伝子の同定? (2010)
  • Author(s): 北本光、佐々木健、三浦郁夫
  • Organizer: 日本爬虫両生類学会第49回大会
  • Place of Presentation: 横浜(神奈川)
  • Year and Date: 20101009-10
• [Presentation] アカハライモリの秋から春をまたぐ多重交配について-両季節の精子が受精に利用されている遺伝学的証拠- (2010)
  • Author(s): 秋山繁治、小泉雄紀、三浦郁夫
  • Organizer: 日本爬虫両生類学会第49回大会
  • Place of Presentation: 横浜(神奈川)
  • Year and Date: 2010-09-10
• [Presentation] 野生ガエルにみられた変態異常 (2009)
  • Author(s): 三浦郁夫、藤谷武史、田上正隆、小泉雄紀、大谷浩己
  • Organizer: 日本環境ホルモン学会第12回年会
  • Place of Presentation: 本郷(東京)
  • Year and Date: 20091207-08
• [Presentation] ツチガエルの性染色体は2重の起源をもつ (2009)
  • Author(s): 三浦郁夫、大谷浩己、尾形光昭
  • Organizer: 染色体学会第60回年会
  • Place of Presentation: 松江(島根)
  • Year and Date: 20091112-14
• [Presentation] ツチガエル性染色体の二重起源と地域分化 (2009)
  • Author(s): 三浦郁夫、大谷浩己、尾形光昭
  • Organizer: 日本爬虫両生類学会第48回年会
  • Place of Presentation: 天理(奈良)
  • Year and Date: 20091107-08
• [Presentation] 性染色体構成が異なるツチガエルの集団における性染色体の起源と分化過程について (2009)
  • Author(s): 宇野好宣、西田千鶴子、大島裕希、横山聡、三浦郁夫、中村正久、松田洋一
  • Organizer: 日本進化学会第11回
  • Place of Presentation: 札幌(北海道)
  • Year and Date: 20090902-04
• [Presentation] The W chromosome evolutionand sex-linked gene expression in theJapanese frog Rana rugosa (2009)
  • Author(s): Miura, I., Ezaz, T., Ohtani, H., Uno, Y., Nishida, N., Matsuda, Y., and Graves, JAM.
  • Organizer: Fifthinternational syumposium on thebiology of vertebrate sexdetermination
  • Place of Presentation: Kona, Hawaii, USA
  • Year and Date: 20090420-24
• [Book] Molecular characterization of ZW sexchromosomes and the prototype chromosomein the frog Rana rugosa, Advances inchromosome sciences (2009)
  • Author(s): Miura I, Ezaz T, Ohtani H, and GravesJAM
  • Total Pages: vol.3 : 101
• [Book] The Wchromosome evolution and sex-linked geneexpression in the Japanese frog Rana rugosa (2009)
  • Author(s): Miura I, Ezaz T, Ohtani H, Uno Y, Nishida C, Matsuda Y, and Graves JAM
  • Total Pages: 123-140
  • Publisher: Nova Science Publishers Inc