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2011 Fiscal Year Final Research Report

Study on molecular co-evolution of enzyme and substrate

Research Project

  • PDF
Project/Area Number 21570240
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Evolutionary biology
Research InstitutionSophia University

Principal Investigator

YASUMASU Shigeki  上智大学, 理工学部, 教授 (00222357)

Co-Investigator(Renkei-kenkyūsha) NISIDA Mutumi  東京大学, 海洋研究所, 教授 (90136896)
NAGATA Koji  東京大学, 大学院・農学生命科学研究科, 准教授 (30280788)
Project Period (FY) 2009 – 2011
Keywords機能進化 / 共進化 / 孵化酵素 / 遺伝子重複 / 卵膜
Research Abstract

In previous phylogenetic analysis, we suggested that fishes included in Elopomorpha, as basal teleosts, possess a single type of hatching enzyme genes, and that fishes in Otocephala and Euteleostei gain two types of hatching enzyme genes called clade I and II genes by duplication. The Elopomorph hatching enzyme and the clade I enzyme swell the egg envelope by cleaving the N-terminal regions of ZP proteins, while the clade II enzyme solubilizes the swollen envelope by cleaving the two specific sites at N-terminal of ZP domain and the middle region on the ZP domain. In the evolutionary scenario, our findings support that the clade I enzymes inherit the ancestral enzyme function, and the clade II enzymes gain a new function during evolution to Otocephala and Euteleostei. Our study also suggested that a change in the egg envelope protein during evolution strongly contributed to the acquisition of a new function of euteleostean clade II enzyme.

  • Research Products

    (12 results)

All 2011 2010

All Journal Article (6 results) (of which Peer Reviewed: 6 results) Presentation (5 results) Book (1 results)

  • [Journal Article] Hatching enzyme of Japanese eel Anguilla japonica and the possible evolution of the egg envelope digestion mechanism2011

    • Author(s)
      Sano K, Kawaguchi M, Yoshikawa M, Kaneko T, Tanaka T, Iuchi I and Yasumasu S
    • Journal Title

      FEBS J

      Volume: 278 Pages: 3711-3723

    • DOI

      DOI:10.1111/j.1742-4658.2011.08289.x

    • Peer Reviewed
  • [Journal Article] Conservation of the egg envelope digestion mechanism of hatching enzyme in euteleostean fishes2010

    • Author(s)
      Kawaguchi M, Yasumasu S, Shimizu A, Sano K, Iuchi I, Nishida M
    • Journal Title

      FEBS J

      Volume: 277 Pages: 4973-4987

    • Peer Reviewed
  • [Journal Article] Evolution of the teleostean zona pellucida gene inferred from the egg envelope protein genes of the Japanese eel, Anguilla japonica2010

    • Author(s)
      Sano K, Kawaguchi M, Yoshikawa M, Iuchi I, Yasumasu S
    • Journal Title

      FEBS J

      Volume: 277 Pages: 4674-4684

    • Peer Reviewed
  • [Journal Article] Intronloss evolution of hatching enzyme gene in Teleostei2010

    • Author(s)
      Kawaguchi M, Hiroi J, Miya M, Nishida M, Iuchi I, Yasumasu S
    • Journal Title

      BMC Evol Biol

      Volume: 27 10 Pages: 260

    • Peer Reviewed
  • [Journal Article] Crystal structure of zebrafish hatching enzyme 1 from the zebrafish Danio rerio2010

    • Author(s)
      Okada A, Sano K, Nagata K, Yasumasu S, Ohtsuka J, Yamamura A, Kubota K, Iuchi I, Tanokura M
    • Journal Title

      J Mol Biol

      Volume: 8(402) Pages: 865-878

    • Peer Reviewed
  • [Journal Article] Mechanism of egg envelope digestion by hatching enzymes2010

    • Author(s)
      Yasumasu S, Kawaguchi M, Ouchi S, Sano K, Murata K, Sugiyama H, Akema T, Iuchi I
    • Journal Title

      HCE and LCE in medaka, Oryzias latipes

      Volume: 148 Pages: 439-448

    • Peer Reviewed
  • [Presentation] ニシン・骨鰾類の2種の孵化酵素の孵化酵素と卵膜の分子共進化卵膜分解機構2011

    • Author(s)
      佐野香織・川口眞理・井内一郎・安増茂樹
    • Organizer
      第82回日本動物学会
    • Place of Presentation
      旭川市大雪クリスタルホール
    • Year and Date
      2011-09-22
  • [Presentation] 孵化酵素と卵膜の分子共進化2011

    • Author(s)
      川口眞理・西田睦・安増茂樹
    • Organizer
      第13回日本進化学会
    • Place of Presentation
      京都大学
    • Year and Date
      2011-07-30
  • [Presentation] タンパク質機能の進化:魚類孵化酵素による卵膜分解メカニズム2011

    • Author(s)
      佐野香織・川口眞理・安増茂樹
    • Organizer
      13回日本進化学会
    • Place of Presentation
      京都大学
    • Year and Date
      2011-07-30
  • [Presentation] Evolution of protein function : the mechanism of egg envelope digestion by fish hatching enzymes as a model2011

    • Author(s)
      Kaori Sano, Mari Kawaguchi, and Shigeki Yasumasu
    • Organizer
      Annual Conference of Society for Molecular Biology and Evolution
    • Place of Presentation
      京都大学
    • Year and Date
      2011-07-27
  • [Presentation] アユ孵化酵素遺伝子群のゲノム構造の探査2011

    • Author(s)
      野村健太郎・川口眞理・竹花佑介・成瀬清・安増茂樹
    • Organizer
      第64回日本動物学会関東支部大会
    • Place of Presentation
      東邦大学習志野キャンパス
    • Year and Date
      2011-03-17
  • [Book] Molecular evolution of teleostean hatching enzymes and their egg envelope digestion mechanism : an aspect of co-evolution of protease and substrate. In Medaka : A Model for Organogenesis, Human Disease and Evolution2011

    • Author(s)
      Yasumasu S, Sano K, Kawaguchi M
    • Total Pages
      361-374
    • Publisher
      Springer

URL: 

Published: 2013-07-31  

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