2011 Fiscal Year Final Research Report
Molecular mechanisms of co-evolution of mitochondrial genetic codes and tRNA in the evolution of animals
| Project/Area Number |
21570242
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Evolutionary biology
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| Research Institution | The University of Tokyo (2010-2011)
National Institute of Advanced Industrial Science and Technology (2009) |
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Principal Investigator |
WATANABE Kimitsuna 東京大学, 大学院・農学生命科学研究科, 名誉教授 (00134502)
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| Co-Investigator(Kenkyū-buntansha) |
YOKOBORI Shin-ichi 東京薬科大学, 生命科学部, 講師 (40291702)
SHIGI Naoki (独)産業技術総合研究所, バイオメディシナル情報研究センター, 研究員 (20392623)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 遺伝暗号 / ミトコンドリア / tRNA / 修飾塩基 / 分子進化 |
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| Research Abstract |
In animal mitochondria, distribution of the non-universal codons(relationship between codon and amino acid) has been clarified in all animal phyla. However, relationship between codon and anticodon has not clarified except for Vertebrata and Nematoda. In order to elucidate a whole image for the decoding mechanism of genetic code, we isolated the relevant tRNAs from a tunicate(ascidian) and a mollusc(squid) and analysed them by using LC/MS. By doing so, we clarified the relationship. We found 5-taurinomethyluridine(. m5U) in the first position of ascidian tRNATrp, tRNAMet and tRNAGly, and squid tRNATrp and concluded that. m5U-bearing anticodons decode A-and G-ending codons.
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[Journal Article] Taurine-containing uridine modifications in tRNA anticodons are required to decipher non-universal genetic codes in ascidian mitochondria2011
Author(s)
Suzuki, T., Miyauchi, K., Suzuki, T., Yokobori, S., Shigi, N., Kondow, A., Takeuchi, N., Yamagishi, A. and Watanabe, K.
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Journal Title
J. Biol. Chem.
Volume: 286
Pages: 35494-35498
Peer Reviewed
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[Journal Article] Male death resulting from hybridization between subspecies of the gypsy moth2011
Author(s)
Higashiura, Y., Yamaguchi, M., Ishihara, M., Ono, N., Tsukagoshi, H., Yokobori, S., Tokishita, S., Yamagata, H. and Fukatsu, T.
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Journal Title
Lymantria disapr. Heredity
Volume: 106
Pages: 603-613
Peer Reviewed
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[Presentation] Unique characteristics of ascidian mitochondrial tRNAs involved in deciphering the non-universal genetic codes and an initiator tRNA corresponding to AUG codon2011
Author(s)
Suzuki, T., Miyauchi, K., Suzuki, T., Yokobori, S., Shigi, N., Kondow, A., Takeuchi, N., Yamagishi, A. and Watanabe, K.
Organizer
RNA2011
Place of Presentation
京都
Year and Date
2011-06-17
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[Presentation] Complete nucleotide sequences of mitochondrial genomes of two salps Ritteriella amboinensis and R. picteri2009
Author(s)
Ohsone, Y., Wada, H., Nishikawa, J., Kurabayashi, A., Hirose, E., Yamagishi, A. and Yokobori, S.
Organizer
The 5th International Tunicate Meeting
Place of Presentation
Okinawa
Year and Date
2009-06-22
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[Presentation] Molucular phylogeny of Urochordata(Tunicata) based on complete mitochondrial genome sequences and 18S rRNA gene sequences.2009
Author(s)
Yokobori, S., Kurabayashi, A., Nishikawa, J., Ohsone, Y., Yamagishi, A. and Hirose, E.
Organizer
The 5th International Tunicate Meeting
Place of Presentation
Okinawa
Year and Date
2009-06-21
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