2011 Fiscal Year Final Research Report
Genomic analysis of the S-locus region and identification of the S determinant in wild barley, Hordeum bulbosum
| Project/Area Number |
21580006
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Breeding science
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| Research Institution | Mie University |
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Principal Investigator |
KAKEDA Katsuyuki 三重大学, 大学院・生物資源学研究科, 教授 (50221867)
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| Co-Investigator(Renkei-kenkyūsha) |
KOMATSUDA Takao 独立行政法人農業生物資源研究所, 基盤研究領域植物ゲノム研究ユニット, 上級研究員 (60370657)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 自家不和合性 / イネ科 / S遺伝子 / ゲノム解析 |
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| Research Abstract |
Hordeum bulbosum, a wild species of barley, possesses self-incompatibility(SI) controlled by unlinked two loci, S and Z. To search for a new candidate gene encoding the pollen S determinant, we conducted sequence analysis of the genomic region around the HPS10 gene, a promising candidate of the pistil S gene. DNA sequences of genomic clone contigs obtained from S1 and S3 haplotypes(50kb and 67kb, respectively) were completely determined and then subjected for prediction of genes and expression analysis. The results showed the presence of polymorphic genomic regions around HPS10 gene between two S haplotypes, where several genes expressed in reproductive tissues were detected. Furthermore, proteome analysis of the pollen proteins combined with the above-mentioned sequence data newly identified putative pollen proteins that may function as a recognition molecule of the SI.
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