2011 Fiscal Year Final Research Report
Analysis of structure and function of RPS4 and RRS1 proteins
| Project/Area Number |
21580060
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Plant pathology
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| Research Institution | 岡山県農林水産総合センター (2011)
Research Institute for Biological Science Okayama (2009-2010) |
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Principal Investigator |
NARUSAKA Yoshihiro 岡山県農林水産総合センター, 生物科学研究所, 研究員 (20335459)
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| Co-Investigator(Renkei-kenkyūsha) |
KUBO Yasuyuki 京都府立大学, 農学研究科, 教授 (80183797)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 抵抗性遺伝子 / 遺伝子対遺伝子説 / シグナル伝達 / 植物免疫 / シロイヌナズナ / 炭疽病 / 青枯病 / トマト斑葉細菌病菌 |
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| Research Abstract |
We found that both Arabidopsis RRS1 and RPS4 are required for resistance to Colletotrichum higginsianum, Ralstonia solanacearum and Pseudomonas syringae pv. tomato strain DC3000 expressing avrRps4.These two adjacent R genes confer resistance, in tandem or individually, to three distinct pathogens with very different infection strategies and virulence mechanisms. The comparison of amino acid sequences of the RPS4 alleles from twenty ecotypes revealed the protein sequences were highly similar. On the contrary, we found several variations in the LRR domain and C-terminal region of RRS1 by comparing twenty ecotypes. Natural variation in receptor-type R proteins often occurs in their LRR domain, typically at the solvent exposedβ-strand/β-turn structure. The strong selection pressure at the LRR domain suggests that this is the domain directly binds to the pathogen determinants that are evolving fast. We introduced some amino acid changes into RPS4 and RRS1, and we revealed the structure and function of RPS4 and RRS1 proteins.
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Research Products
(35 results)
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[Presentation] デュアル抵抗性蛋白質システムによる病原体認識機構の解明:RPS4とRRS1遺伝子の作物への導入2012
Author(s)
鳴坂真理,白須賢,久保康之,白石友紀,畠山勝徳,平井正良,河本晃一,江面浩,高野義孝,岩渕雅樹,鳴坂義弘
Organizer
第53回日本植物生理学会年会
Place of Presentation
京都
Year and Date
20120316-18
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[Presentation] A dual Resistance-gene system confers resistance against fungal and bacterial pathogens in transgenic Brassica rapa2011
Author(s)
Narusaka, M., Shirasu, K., Kubo, Y., Shiraishi, T., Hatakeyama, K., Hirai, T., Kawamoto, K., Ezura, H., Mukaihara, T., Iwabuchi, M., Narusaka, Y
Organizer
第34回日本分子生物学会年会
Place of Presentation
横浜
Year and Date
20111213-16
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[Presentation] RRS1 and RPS4 provide a dual Resistance-gene system against fungal and bacterial pathogens2011
Author(s)
Narusaka, Y., Narusaka, M., Shirasu, K., Kubo, Y., Shiraishi, T., Iwabuchi, M
Organizer
第34回日本分子生物学会年会
Place of Presentation
横浜
Year and Date
20111213-16
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[Presentation] デュアルR-遺伝子による病原体認識機構:RPS4とRRS1遺伝子の作物への導入2011
Author(s)
鳴坂真理,白須賢,久保康之,白石友紀,畠山勝徳,平井正良, Seung Won Kang,河本晃一,江面浩,岩渕雅樹,鳴坂義弘
Organizer
第52回日本植物生理学会年会
Place of Presentation
仙台
Year and Date
20110320-22
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