2011 Fiscal Year Final Research Report
An improvement of insect cell-free protein synthesis system
| Project/Area Number |
21580069
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied entomology
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| Research Institution | Okinawa National College of Technology |
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Principal Investigator |
ITO Masaaki 沖縄工業高等専門学校, 生物資源工学科, 教授 (00395659)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 昆虫利用・機能開発 / 無細胞タンパク質合成系 |
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| Research Abstract |
In the present study, we analyzed an enhancer sequence composed of Malacosma Neustria nucleopolyhedrovirus polyhedorin 5'-UTR for improvement of insect cell-free protein synthesis system. As a result, we found important sequence of 16 base length. A protein synthesis activity using twice repeat of the sequence as enhancer was 1.4-fold greater than that of using usual enhancer. Furthermore, we constructed cell-free expression and analysis systems for organic solvent resistant tyrosinase(metal protein) and thermostable laccase(membrane-binding protein)
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[Journal Article] Development of an insect cell-free system2010
Author(s)
Suzuki, T., Ezure, T., Shikata, M., Ito, M., Ando, E., Utsumi, T., Nishimura, O., and Tsunasawa, S
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Journal Title
Current Pharmaceutical Biotechnology
Volume: 11
Pages: 279-284
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[Book] A cell-free protein synthesis system from insect cells in Cell-free Protein Production, Methods in Molecular Biology2010
Author(s)
Ezure, T., Suzuki, T., Shikata, M., Ito, M., and Ando, E.
Total Pages
31-42
Publisher
Humana Press
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[Book] An insect cell-free system for recombinant protein expression using cDNA resources in Reverse Chemical Genetics, Methods in Molecular Biology2009
Author(s)
Suzuki, T., Ezure, T., Ito, M., Shikata, M., and Ando, T.
Total Pages
97-108
Publisher
Humana Press
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