2011 Fiscal Year Final Research Report
Functional analysis of genes from an anhydrobiotic chironomid, Polypedilum vanderplanki, using the transgenic fly system
| Project/Area Number |
21580071
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied entomology
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| Research Institution | Kyoto Institute of Technology |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
YAMAMOTO Masatoshi 京都工芸繊維大学, ショウジョウバエ遺伝資源センター, 教授 (10142001)
KIKAWADA Takahiro 農業生物資源研究所 (60414900)
OKUDA Takashi 農業生物資源研究所 (60414893)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 乾燥耐性 / ネムリユスリカ / トレハロース / LEAタンパク質 / キイロショウジョウバエ |
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| Research Abstract |
To analysis the functions of trehalose and four LEA proteins in the dried state of an anhydrobiotic chironomid, Polypedilum vanderplanki larva, desiccation-related genes were introduced into Drosophila melanogaster. The adult flies expressing four LEA protein genes had no additional tolerance for desiccation ; moreover, PvLEA1-expressed male fly presented the sensitivity for desiccation. This suggests that PvLEA proteins concern with desiccation tolerance of P. vanderplanki indirectly.
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[Journal Article] Identification of anhydrobiosis-related genes from an expressed sequence tag database in the crypto biotic midge Polypedilum vanderplanki(Diptera ; Chironomidae).2010
Author(s)
Cornette, R., Kanamori, Y., Watanabe, M., Nakahara, Y., Gusev, O., Mitsumasu, K., Kadono-Okuda, K., Shimomura, M., Mita, K., Kikawada, T, Okuda, T
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Journal Title
J Biol Chem
Volume: Vol.285
Pages: 35889-35899
Peer Reviewed
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[Journal Article] Enzymatic control of anhydrobiosis-related accumulation of trehalose in the sleeping chironomid, Polypedilum vanderplanki.2010
Author(s)
Mitsumasu, K., Kanamori, Y., Fujita, M., Iwata, K., Tanaka, D., Kikuta, S., Watanabe, M., Cornette, R., Okuda, T., Kikawada, T.
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Journal Title
FEBS J.
Volume: Vol.27
Pages: 4215-4228
Peer Reviewed
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[Journal Article] Desiccation-induced structuralization and glass formation of group 3 late embryogenesis abundant protein model peptides2010
Author(s)
Shimizu, T., Kanamori, Y., Furuki, T., Kikawada, T., Okuda, T., Takahashi, T., Mihara, H., Sakurai, M.
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Journal Title
Biochemistry
Volume: Vol.40(6)
Pages: 1093-1104
Peer Reviewed
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[Journal Article] The trehalose transporter 1 gene sequence is conserved in insects and encodes proteins with different kinetic properties involved in trehalose import into peripheral tissues2010
Author(s)
Kanamori, Y., Saito, A., Hagiwara-Komoda, Y., Tanaka, D., Mitsumasu, K., Kikuta, S., Watanabe, M., Cornette, R., Kikawada, T., Okuda, T.
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Journal Title
Insect Biochem Mol Biol
Volume: Vol.40
Pages: 30-37
Peer Reviewed
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