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2011 Fiscal Year Final Research Report

Functional characterization of two-component high-affinity nitrate transport system

Research Project

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Project/Area Number 21580076
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Plant nutrition/Soil science
Research InstitutionNiigata University

Principal Investigator

SUEYOSHI Kuni  新潟大学, 自然科学系, 准教授 (10216278)

Project Period (FY) 2009 – 2011
Keywordsオオムギ / 硝酸イオン / 膜輸送 / トランスポーター
Research Abstract

In barley, low level of external nitrate is absorbed by high affinity transport system(HATS) which is composed of two proteins, HvNRT2 and HvNAR2. In this study, it was suggested that two proteins make complex in the isolated plasma membrane and co-localized at out most layer of the cells in root cortex. In addition, Bimolecular fluorescence complementation analysis showed that HvNRT2 and HvNAR2 interacts in the plasma membrane of the native plant cells.

  • Research Products

    (7 results)

All 2012 2011 2010 2009 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (5 results) Remarks (1 results)

  • [Journal Article] Two-component high-affinity nitrate transport system in barley : membrane localization, protein expression in roots and a direct protein-protein interaction2009

    • Author(s)
      Ishikawa, Y. Ito, Y. Sato, Y. Fukaya, M. Takahashi, H. Morikawa, N. Ohtake, T. Ohyam and K. Sueyoshi
    • Journal Title

      Plant Biotechnol

      Volume: vol.26 Pages: 197-205

    • Peer Reviewed
  • [Presentation] 植物トランスポーター研究の新しい展開2012

    • Author(s)
      末吉邦
    • Organizer
      第4回ストレス科学シンポジウム
    • Place of Presentation
      倉敷,岡山
    • Year and Date
      2012-03-08
  • [Presentation] オオムギ低親和性硝酸トランスポーターの機能解析2011

    • Author(s)
      石川伸二・園部和幸・三谷奈見季・馬建鋒・原田久富美・大山卓爾・末吉邦
    • Organizer
      日本土壌肥料学会2011年つくば大会
    • Place of Presentation
      つくば,茨城
    • Year and Date
      2011-08-09
  • [Presentation] Nitrate transporters in barley2011

    • Author(s)
      Sueyoshi K
    • Organizer
      3rd Japan-China Joint Workshop on Plant Nutrition
    • Place of Presentation
      倉敷,岡山
    • Year and Date
      2011-03-27
  • [Presentation] Two-component high-affinity nitrate transport system in barley : Localization in roots and protein-protein interaction in plant cells2010

    • Author(s)
      Ishikawa, S, Ohishi M, Yamaji N, Ma JF, Ohtake N, Ohyama T, Sueyoshi K
    • Organizer
      1st International Symposium on the Nitrogen Nutrition of Plants
    • Place of Presentation
      犬山,愛知
    • Year and Date
      2010-07-27
  • [Presentation] Nitrate uptake, transport and accumulation in higher plants2009

    • Author(s)
      Sueyoshi K, Ohyama T
    • Organizer
      2nd International Meeting of IPM
    • Place of Presentation
      ハノイ,ベトナム
    • Year and Date
      2009-12-09
  • [Remarks]

    • URL

      http://www.agr.niigata-u.ac.jp/~sueyoshi/index.html

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Published: 2013-07-31  

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