2011 Fiscal Year Final Research Report
Clarification of horizontal gene transfer mechanism in Streptomyces
Project/Area Number |
21580090
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Applied microbiology
|
Research Institution | Shinshu University |
Principal Investigator |
|
Project Period (FY) |
2009 – 2011
|
Keywords | 微生物遺伝 / ゲノム進化 / 放線菌 / 接合伝達 / TraB / 局在 / 発現 |
Research Abstract |
The conjugative transfer of the small plasmid takes a main role in horizontal gene transfer in Streptomyces. The small criptic plasmid, pSN22, from Streptomyces nigrifaciens can mobilize the host chromosome along with the plasmid transfer. We identified both the TraB protein is essential for the plasmid and genome transfer through cytoplasmic membrane and the ATP activity is essential for the DNA channel function. In addition the elements acted in trans, we also identified cis element essential for the plasmid transfer and determined the distinctive structure.
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