2011 Fiscal Year Final Research Report
Library construction of decarboxylases catalyzing carbon dioxide fixation and their application for the production of various aromatic carboxylic acids
| Project/Area Number |
21580091
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Gifu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIDA Toyokazu 岐阜大学, 工学部, 准教授 (90220657)
MITSUKURA Koichi 岐阜大学, 工学部, 助教 (70324283)
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| Co-Investigator(Renkei-kenkyūsha) |
MATSUDA Tomoko 東京工業大学, 大学院・生命理工学研究科, 講師 (10319494)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 発酵生産 |
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| Research Abstract |
We have found several aromatic or heterocyclic carboxylic acid decarboxylases catalyze Kolbe-Schmitt reaction. We characterized them and the high expression of each gene and the optimization of reaction conditions of carbon dioxide fixation were studied. We constructed the library of these enzymes and proposed the production process of various carboxylic acids. These enzymes compose of hetero-oligomer and a small molecule subunit participates as a stable factor, which were very important for the further application of these enzymes.
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