Mechanisms of oxidative protein folding in the periplasm of E. coli

2011 Fiscal Year Final Research Report

• Project/Area Number: 21580092
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: KADOKURA Hiroshi 奈良先端科学技術大学院大学, バイオサイエンス研究科, 研究員 (70224558)
• Project Period (FY): 2009 – 2011
• Keywords: ジスルフィド結合 / 立体構造形成 / 分泌 / DsbA

Research Abstract

Disulfide bonds, formed between pairs of cysteines, are important for the folding,activity, and stability of many secretory and membrane proteins. In a bacterium Escherichia coli, an enzyme called DsbA introduces disulfide bonds into folding proteins in the periplasm. In this study, I detected, in vivo, the intermediates of disulfide bond formation that were formed between DsbA and its substrates. Detailed characterization of the intermediates revealed a number of new insights into protein folding in this cellular compartment.

Research Products

• [Journal Article] rearrangement triggered by translocon assembly controls lipopolysaccharide export (2012)
  • Author(s): Chng, S.S., Xue, M., Garner, R.A.,Kadokura, H., Boyd, D., Beckwith, J.,Kahne, D. Disulfide
  • Journal Title: Science Volume: 337 Pages: 1665-1668
  • Peer Reviewed
• [Journal Article] Mechanisms of oxidative protein folding in the bacterial cell envelope. Antioxid (2010)
  • Author(s): Kadokura, H., Beckwith, H.
  • Journal Title: Redox Signal Volume: 13 Pages: 1231-1246
  • Peer Reviewed
• [Journal Article] The L-cysteine/L-cystine shuttle system provides reducing equivalents to the periplasm in Escherichia coli (2010)
  • Author(s): Ohtsu, I., Wiriyathanawudhiwong, N.,Morigasaki, S., Nakatani, T., Kadokura H., Takagi H.
  • Journal Title: J. Biol. Chem Volume: 285 Pages: 17479-17487
  • Peer Reviewed
• [Journal Article] 分泌タンパク質にジスルフィド結合を形成する仕組み:ジスルフィド結合形成反応中間体の検出 (2010)
  • Author(s): 門倉 広
  • Journal Title: 化学と生物 Volume: 48 Pages: 695-705
• [Journal Article] Detecting folding intermediates of a protein as it passes through the bacterial translocation channel (2009)
  • Author(s): Kadokura, H., Beckwith, J.
  • Journal Title: Cell Volume: 138 Pages: 1164-1173
  • Peer Reviewed
• [Presentation] 哺乳動物細胞小胞体におけるジスルフィド結合形成を解析する為の新規レポーターの作成 (2013)
  • Author(s): 門倉 広、河野憲二
  • Organizer: 日本農芸化学会2012 年度大会
  • Place of Presentation: 京都女子大学
  • Year and Date: 2013-03-23
• [Presentation] 反応中間体の解析から見えてきた、生体内における分泌蛋白質の酸化的フォールディングの仕組み (2011)
  • Author(s): 門倉 広、Jon Beckwith、河野憲二
  • Organizer: 第84回 日本生化学会大会
  • Place of Presentation: 京都国際会館
  • Year and Date: 2011-09-21
• [Presentation] Developing a genetic assay for the analysis of disulfide bond formation in the ER of mammalian cells (2011)
  • Author(s): Kadokura, H.
  • Organizer: Microbial Genetics and Genomics V
  • Place of Presentation: Harvard Medical School in Boston; Oral
  • Year and Date: 2011-05-01
• [Presentation] Mechanisms of oxidative protein folding in vivo: Novel features revealed by the analysis of disulfide- linked enzyme-substrate complexes (2010)
  • Author(s): Kadokura, H.
  • Organizer: The 3rd International Symposium on Protein Community
  • Place of Presentation: Hotel Nikko Nara;Oral, Invited presentation
  • Year and Date: 2010-09-14
• [Presentation] 生体内で分泌蛋白質にジスルフィド結合を形成する仕組み:ジスルフィド結合形成反応中間体の検出およびその解析 (2010)
  • Author(s): 門倉 広
  • Organizer: 第10回日本蛋白質学会大会
  • Place of Presentation: 札幌コンベンションセンター
  • Year and Date: 2010-06-16
• [Presentation] 生体内における分泌タンパク質ジスルフィド結合形成反応中間体の解析 (2010)
  • Author(s): 門倉 広、河野憲二、Jon Beckwith
  • Organizer: 日本農芸化学会2010 年度大会
  • Place of Presentation: 東京大学駒場キャンパス
  • Year and Date: 2010-03-29
• [Remarks] ホームページアドレス
  • URL: http://bsw3.naist.jp/kouno/kouno.html