2011 Fiscal Year Final Research Report
Studies on the structure and function of enzymes related to C-S bond formation and cleavage of useful naturally-occuring cyclic compounds having sulfur
| Project/Area Number |
21580093
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Tottori University |
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Principal Investigator |
IZUMI Yoshikazu 鳥取大学, 大学院・工学研究科, 名誉教授 (40026555)
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| Co-Investigator(Kenkyū-buntansha) |
MORI Nobuhiro 鳥取大学, 農学部, 教授 (30127469)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 好熱性微生物 / 好熱性酵素 / 環状含硫有機化合物 / ビオチン生合成系酵素 / ジベンゾチオフェン脱硫酵素系 |
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| Research Abstract |
The structure and function of thermophilic enzymes related to C-S bond formation and cleavage of naturally occuring important cyclic compounds were studied for the first time. Targeted enzymes are KAPA synthase(BioF), which is involved in the first step of biotin(vitamin H) synthetic pathway, as the model enzyme related to C-S bond formation pathway, and dibenzothiophene(DBT) monooxigenase(BdsC), which is involoved in the first and second steps of DBT, an air pollutant contained in petroleum, as the model enzyme related to C-S bond cleavage pathway. As for the former enzyme, the temperature properties and substrate specificities were significantly superior to those of known mesophilic organisms. As for the latter enzyme, a useful new reaction was found in terms of application.
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