2011 Fiscal Year Final Research Report
Transcriptional analysis of silica-induced protein in Thermus thermophilus and its application to heterologous protein production
| Project/Area Number |
21580097
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | Kyushu University |
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Principal Investigator |
DOI Katsumi 九州大学, 農学研究院, 講師 (40253520)
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| Co-Investigator(Kenkyū-buntansha) |
TASHIRO Kosuke 九州大学, 大学院・農学研究院, 准教授 (00192170)
FUJINO Yasuhiro 九州大学, 基幹教育院, 助教 (70582659)
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| Project Period (FY) |
2009 – 2011
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| Keywords | Thermus thermophilus / シリカ / ABCトランスポーター / DNAマイクロアレイ / 大量発現系 / 鉄イオン |
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| Research Abstract |
Thermus cells produce the specific protein, a silica-induced protein(Sip), only when they are cultivated in the culture containing supersaturated silica. DNA microarray analysis under the silica-stressed and iron-deficient conditions were performed to detect the signal transduction in sip expression. From the results, Thermus cells may receive the signal of the existence of supersaturated silica as the iron deficiency, and may gather iron ions or other metal cations to their surfaces.
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