Studies on imbalance localization of phospholipids in the cellular membrane

2011 Fiscal Year Final Research Report

• Project/Area Number: 21580099
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Kagoshima University
• Principal Investigator: TAMAKI Hisanori 鹿児島大学, 農学部, 准教授 (20212045)
• Co-Investigator(Renkei-kenkyūsha): MIYASHITA Masahiro 京都大学, 大学院・農学研究科, 助教 (80324664)
• Project Period (FY): 2009 – 2011
• Keywords: アシル転移酵素 / 脂質の不均衡分布 / 酵母 / 細胞膜リン脂質

Research Abstract

In this study, we clarify the relation between imbalance localization of double-bond in the phospholipids and newly found lyso-phospholipid acyltransferases. Two acyltransferases Lpt1 and Slc1 were purified(or partially purified) and substrate specificities were examined. Also total lipids were extracted from lpt1 or slc1 gene disrupted strain and subjected to LC-MS/MS analysis. From these experiments, Two enzymes mainly catalyze introduction of acyl-residue containing double-bond, although substrate preference were different.

Research Products

• [Journal Article] Development of a heat-processing method for koji to enhance its antioxidant activity (2012)
  • Author(s): Okutsu, K., Yoshizaki, Y., Takamine, K., Tamaki, H., Ito, K., and Sameshima, Y.
  • Journal Title: J. Biosci. Bioeng. Volume: 113(3) Pages: 349-354
  • Peer Reviewed
• [Journal Article] The formation of beta-damascenone in sweet potato shochu (2011)
  • Author(s): Yoshizaki, Y., Takamine, K., Shimada, S., Uchihori, K., Okutsu, K., Tamaki, H., Ito, K., and Sameshima Y.
  • Journal Title: J. Inst. Brew Volume: 117 Pages: 217-223
  • Peer Reviewed
• [Journal Article] Role of a PA14 domain in determining substrate specificity of a glycoside hydrolase family 3β-glucosidase from Kluyveromyces marxianus (2010)
  • Author(s): Yoshida, E., Hidaka, M., Fushinobu, S., Koyanagi, T., Minami, H., Tamaki, H., Kitaoka, M., Katayama, T., and Kumagai, H.
  • Journal Title: Biochem. J. Volume: 431(1) Pages: 39-49
  • Peer Reviewed
• [Presentation] 分裂酵母リゾリン脂質アシル転移酵素Slc1の機能解析 (2011)
  • Author(s): 福田竜也
  • Organizer: 第44回酵母遺伝学フォーラム
  • Place of Presentation: 福岡、九大医学部百年講堂
  • Year and Date: 2011-09-06
• [Presentation] 酵母アシルトランスフェラーゼの機能解析 (2010)
  • Author(s): 玉置尚徳
  • Organizer: 第52回日本脂質生化学会
  • Place of Presentation: 群馬、伊香保温泉
  • Year and Date: 2010-06-15
• [Presentation] 酵母新規アシル転移酵素の機能解析 (2009)
  • Author(s): 玉置尚徳
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸、ポートピアホテル
  • Year and Date: 2009-10-24