Development of Pro-biotic Lactic Acid Bacteria Scavenging Environmental Hydroperoxides

2011 Fiscal Year Final Research Report

• Project/Area Number: 21580101
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Tokyo University of Agriculture
• Principal Investigator: NIIMURA Youichi 東京農業大学, 応用生物科学部, 教授 (00180563)
• Project Period (FY): 2009 – 2011
• Keywords: 乳酸菌 / プロバイオティックス / 過酸化脂質 / 過酸化脂質分解

Research Abstract

In order to get lactic acid bacteria scavenging environmental lipid peroxide, we successfully isolated Lactobacillus plantarum P2 strain and purified two lipid peroxide reductases from the bacteria. The enzymes catalyze the reduction of lipid peroxide to its alcohol derivative with NAD(P) H as the preferred electron donor. The gene of the enzyme was cloned and overexpressed in E. coli. The recombinant enzyme has been purified to homogeneity and analyzed to get enzymatic parameters.

Research Products

• [Journal Article] Lactobacillus ozensis sp. nov., a novel lactic acid bacteria isolated from mountain flowers in Oze National Park (2011)
  • Author(s): Kawasaki S, Kurosawa K, Miyazaki M, Sakamoto M, Ohkuma M, Y. Niimura
  • Journal Title: It. J. Syst. Evol.Microbiol Volume: 61巻 Pages: 2435-2438
  • Peer Reviewed
• [Journal Article] Lactobacillus floricola sp. nov., a novel lactic acid bacteria isolated from mountain flowers in Japan (2011)
  • Author(s): Kawasaki S, Kurosawa K, Miyazaki M, Yagi C, Kitajima Y, Tanaka S, Irisawa T, Okada S, Sakamoto M, Ohkuma M, Y. Niimura
  • Journal Title: It. J. Syst. Evol.Microbiol Volume: 61巻 Pages: 1356-1359
  • Peer Reviewed
• [Journal Article] Synechocystis ferredoxin-NADP^+oxidoreductase is capable of functioning as ferric reductase and of driving the Fenton reaction in the absence or presence of free flavin (2011)
  • Author(s): Junichi S., K. Takeda, R. Nishiyama, T. Watanabe, M. Abo, E. Yoshimura, J. Nakagawa, A. Abe, S. Kawasaki, Y. Niimura
  • Journal Title: Biometals Volume: 24巻 Pages: 311-321
  • Peer Reviewed
• [Journal Article] Escherichia coli ferredoxin-NADP^+reductase and oxygen-insensitive NAD(P) H nitroreductase are capable of functioning as ferric reductase and of driving the Fenton reaction (2010)
  • Author(s): K. Takeda, J. Sato, K. Goto, T. Fujita, T. Watanabe, M. Abo, E. Yoshimura, J. Nakagawa, A. Abe, S. Kawasaki, Y. Niimura
  • Journal Title: Biometals Volume: 23巻 Pages: 727-737
  • Peer Reviewed
• [Journal Article] Chlorella vulgaris aldehyde reductase is capable of functioning as ferric reductase and of driving the Fenton react ion in the presence of free flavin (2010)
  • Author(s): J. Sato, K. Takeda, R. Nishiyama, K. Fusayama, T. Arai, T. Sato, T. Watanabe, A. Abe, J. Nakagawa, S. Kawasaki, Y. Niimura
  • Journal Title: Bioscience Biotechnology and Biochemistry Volume: 74巻 Pages: 854-857
  • Peer Reviewed
• [Journal Article] Crystal structure of NADH : rubredoxin oxidoreductase from Clostridium. acetobutylicum : a key component of the dioxygen scavenging system in obligatory anaerobes (2010)
  • Author(s): Nishikawa K., Shomura Y., Kawasaki S., Y. Niimura., H. Yoshiki.
  • Journal Title: Proteins Volume: 78巻 Pages: 1066
  • Peer Reviewed
• [Journal Article] Sodium Acetate Enhances Hydrogen Peroxide Production in Weissella cibaria (2009)
  • Author(s): A. Endo, Y. Futagawa-Endo, S. Kawasaki, Leon M. T. Dicks, Y. Niimura & S. Okada
  • Journal Title: Letters in Applied Microbiology-LAN
  • Peer Reviewed
• [Journal Article] Structure analysis of the flavoredoxin from Desulfovibrio vulgaris Miyazaki F reveals key residues that discriminate the functions and properties of the flavin reductase family (2009)
  • Author(s): N. Shibata, Y. Ueda, D. Takeuchi, Y. Haruyama, S. Kojima, J. Sato, Y. Niimura, M. Kitamura, Y. Higuchi
  • Journal Title: FEBS Journal Volume: 276巻 Pages: 4840-4853
  • Peer Reviewed
• [Journal Article] O_2 and reactive oxygen species detoxification complex, composed of O_2-responsive NADH : rubredoxin oxidoreductase-flavoprotein A2-desulfoferrodoxin operon enzymes, rubperoxin, and rubredoxin, in Clostridium acetobutylicum (2009)
  • Author(s): Kawasaki, S, Y. Sakai, T. Takahashi, I. Suzuki and Y. Niimura
  • Journal Title: Appl. Environ. Microbiol. Volume: 75巻 Pages: 1021-1029
  • Peer Reviewed
• [Presentation] 微生物の過酸化物分解酵素の多様性 (2011)
  • Author(s): 新村洋一、望月大地、新井俊晃、大山修平、佐藤純一、川崎信治、渡邊昭夫
  • Organizer: 生体触媒化学シンポジウム
  • Place of Presentation: 慶應義塾大学薬学部
  • Year and Date: 2011-12-21
• [Presentation] 過酸化物分解する乳酸菌の開発 (2011)
  • Author(s): 渡邊昭夫、大山修平、佐藤純一、新村洋一
  • Organizer: 生体触媒化学シンポジウム
  • Place of Presentation: 慶應義塾大学薬学部
  • Year and Date: 2011-12-21