2011 Fiscal Year Final Research Report
Elucidation of reaction mechanism of novel biosynthetic enzymes for derivatization of reveromycin
| Project/Area Number |
21580104
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Applied microbiology
|
|---|
| Research Institution | The Institute of Physical and Chemical Research |
|---|
Principal Investigator |
TAKAHASHI Shunji 独立行政法人理化学研究所, 化学情報・化合物創製チーム, チームヘッド (30311608)
|
|---|
| Project Period (FY) |
2009 – 2011
|
| Keywords | 放線菌 / 天然化合物 / ポリケチド / リベロマイシンA / スピロアセタール化合物 / 生合成 |
|---|
| Research Abstract |
We disrupted reveromycin biosynthetic gene cluster and analyzed the structure of biosynthetic intermediates isolated from each disruptant. We identified various reveromycin derivatives required for the speculation of its biosynthetic pathway. Moreover, the analysis of biosynthetic mechanisms using RM-A1a(triol intermediate) and recombinant enzymes resulted in the identification of dihydroxy ketone synthase(RevG) and spiroacetal synthase(RevJ), which are involved in stereospecific spiroacetal formation.
|
