2011 Fiscal Year Final Research Report
Evaluation of cDNA synthesis and RNA amplification with highly efficient reverse transcriptases and expansion of their use
| Project/Area Number |
21580110
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2009 – 2011
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| Keywords | 酵素利用学 |
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| Research Abstract |
We generated highly thermostable reverse transcriptases from Moloney murine leukemia virus and avian myeloblastosis virus(E286R/E302K/L435R/D524A and V238R/L388R/D450A, respectively) by site-directed mutagenesis. It is suggested that introduction of positive charges into reverse transcriptases at positions that have been implicated in the interaction with T/P is effective to stabilize reverse transcriptases.
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