Fundamental Research for the synthesis of biorecognition glycosides by enzymatic introduction of oligosaccharides from glycoproteins

2011 Fiscal Year Final Research Report

• Project/Area Number: 21580117
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied biochemistry
• Research Institution: Osaka City University
• Principal Investigator: ITO Kazuo 大阪市立大学, 大学院・理学研究科, 准教授 (20183171)
• Co-Investigator(Renkei-kenkyūsha): MIYAHARA Ikuko 大阪市立大学, 大学院・理学研究科, 准教授 (40271176)
• Research Collaborator: HAMAGUCHI Tasuku , 学生 ; TANIWAKI Satoshi , 学生 ; UMEBAYASHI Hidetada , 学生 ; OKA Yuki , 学生 ; GOTO Mariko , 学生 ; SHINOHARA Kanako , 学生 ; RYU Hao , 学生 ; KAIGUMI Takahiro , 学生 ; TAKATANI Naoya , 学生 ; TAKEUCHI Tomofumi , 学生 ; YOSHIDA Tatsuya , 学生 ; ISHII Maiko , 学生 ; TAKAHASI Go , 学生
• Project Period (FY): 2009 – 2011
• Keywords: 糖鎖 / 生体認識 / 酵素 / 配糖体

Research Abstract

Expression system and purification method of recombinant N-linked oligosaccharide-releasing enzyme was established. The recombinant enzyme released oligosaccharides from various glycoproteins. Furthermore, it transferred the released oligosaccharides to form potential bio-recognition glycosides. Analysis of mutant recombinant enzyme showed the functional area on the enzyme. Furthermore, Crystallization of the recombinant enzyme succeeded for X-ray structural analysis.

Research Products

• [Journal Article] Asparagine-linked oligosaccharide-releasing enzymes produced by basidiomycetes : Tasuku Hamaguchi (2011)
  • Author(s): Yukako Inoue, Tsukasa Ito, Kanako Shinohara, Satoshi Taniwaki, Nobuhiro Kaizumi, Hiromoto Hisada, Yoji Hata, Tipaporn Limpaseni, Piamsook Pongsawasdi, and Kazuo Ito
  • Journal Title: Oyotositukagaku Volume: 1 Pages: 159-167
  • Peer Reviewed
• [Journal Article] Synthesis of epicatechin glucosides by aυ-cyclodextrin glycosyltransferase : Pornpun Aramsangtienchai (2011)
  • Author(s): Warinthorn Chavasiri, Kazuo Ito and Piamsook Pongsawasdi
  • Journal Title: Journal of Molecular Catalysis B : Enzymatic Volume: 73 Pages: 27-34
  • Peer Reviewed
• [Journal Article] Molecular and biochemical characterization of levansucrase from Bacillus licheniformis RN-01.Santhana Nakapong, Rath Pichyangkura (2011)
  • Author(s): Masaru Iizuka, Kazuo Ito and Piamsook Pongsawasdi.
  • Journal Title: The 3^<rd> Biochemistry and Molecular Biology International Conference, Chiang Mai Pages: 6-8
• [Journal Article] Synthesis of a novel prebiotic trisaccharide by a type Iτ-glucosidase from B. licheniformis strain TH4-2 : P. Nimpiboon (2011)
  • Author(s): Santhana Nakapong, Rath Pichyangura, Kazuo Ito, and Piamsook Pongsawasdi
  • Journal Title: Process Biochem Volume: 46 Pages: 448-457
  • Peer Reviewed
• [Journal Article] Synthesis of cellobiose-containing oligosaccharides by Intermolecular transglucosylation of cyclodextrin glycosyltransferase from Paenibacillus sp A11 : Wannapa Wongsangwattana (2010)
  • Author(s): Jarunee Kaulpiboon, Kazuo Ito, and Piamsook Pongsawasdi
  • Journal Title: Process Biochemistry Volume: 45 Pages: 947-953
  • Peer Reviewed
• [Journal Article] Purification, Characterization and Molecular Cloning of a Novel Endo-υ-N-acetylglucosaminidase from the Basidiomycete, Flammulina velutipes (2010)
  • Author(s): Tasuku Hamaguchi, Tsukasa Ito, Yukako Inoue, Tipaporn, Limpaseni, Piamsook Pongsawasdi, and Kazuo Ito
  • Journal Title: Glycobiology Volume: 1 Pages: 420-432
  • Peer Reviewed
• [Presentation] 担子菌Agaricus bisporus由来エンド-.-N-アセチルグルコサミニダーゼABの遺伝子クローニングに関する研究 (2012)
  • Author(s): 高橋剛生、谷脇聡、浜口祐、岡夕貴、Kuakarun Krusong、Jarunee Kaulpiboon、TipapornLimpaseni、Piamsook Pongsawasdi、伊藤和央
  • Organizer: 2012年日本農芸化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-25
• [Presentation] 担子菌Flammulina velutipes由来エンド-.-N-アセチルグルコサミニダーゼFVのプロセシング酵素の精製とその同定 (2012)
  • Author(s): 海住宜広、浜口祐、後藤眞璃子、KuakarunKrusong、Jarunee aulpiboon Tipaporn Limpaseni、Piamsook Pongsawasdi、伊藤和央
  • Organizer: 2012年日本農芸化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-25
• [Presentation] 担子菌Flammulina velutipes由来エンド-.-N-アセチルグルコサミニダーゼFV遺伝子の酵母での発現とX線結晶構造解析 (2012)
  • Author(s): 浜口祐、海住宜広、篠原かな子、宮原郁子、田中里佳、Kuakarn Kursong、Jarnee Kaurpiboon、Tipaporn Limpaseni、Piamsook Pongsawasdi、伊藤和央
  • Organizer: 2012年日本農芸化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2012-03-25
• [Presentation] 担子菌Flammulina. velutipesにおけるアスパラギン結合型糖鎖遊離酵素のプロセシング酵素 (2011)
  • Author(s): 海住宜広、濱口祐、久田博元、秦洋二、Kuakarun Krusong、Jarunee Kaulpiboon、Tipaporn Limpaseni、Piamsook Pongsawasdi、伊藤和央
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2011-09-22
• [Presentation] 担子菌由来エンド-.-N-アセチルグルコサミニダーゼFV遺伝子の酵母での発現と発現酵素の性質 (2011)
  • Author(s): 浜口祐、海住宜広、篠原かな子、Kuakarn Kursong、Jarnee Kaurpiborn、Tipaporn Limpaseni、Piamsook Pongsawasdi、伊藤和央
  • Organizer: 第84回日本生化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2011-09-22
• [Presentation] 担子菌Flammulina. velutipesにおけるアスパラギン結合型糖鎖遊離酵素のプロセシング (2011)
  • Author(s): 海住宜広、濱口祐、久田博元、秦洋二、Kuakarun Krusong、Jarunee Kaulpiboon、Tipaporn Limpaseni、Piamsook Pongsawasdi、伊藤和央
  • Organizer: 第58回日本生化学会近畿支部例会
  • Place of Presentation: 大阪
  • Year and Date: 2011-05-21
• [Presentation] Molecular and biochemical characterization of levansucrase from Bacillus licheniformis RN-01 (2011)
  • Author(s): Nakapong, S., Pichyangkura, R., Iizuka, M., Ito, K., and Pongsawasdi, P.
  • Organizer: The 3^<rd> Biochemistry and Molecular Biology International Conference
  • Place of Presentation: Chiang Mai, Thailand
  • Year and Date: 2011-04-20
• [Presentation] 担子菌Agaricus bisporus由来エンド-.-N-アセチルグコサミニダーゼABの精製とその特異性 (2011)
  • Author(s): 谷脇聡、濱口祐、Kuakarun Krusong、Jarunee Kaulpiboon、Tipaporn Limpaseni、Piamsook Pongsawasdi、伊藤和央
  • Organizer: 2011年日本農芸化学会大会
  • Place of Presentation: 京都
  • Year and Date: 2011-03-27
• [Presentation] 担子菌Agaricus bisporus由来エンド-.-N-アセチルグルコサミニダーゼABの精製とその性質 (2010)
  • Author(s): 谷脇聡、濱口祐、Kuakarun Krusong、Jarunee Kaulpiboon、Tipaporn Limpaseni、PiamsookPongsawasdi、伊藤和央
  • Organizer: 第83回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-10
• [Presentation] Comparative study of basidiomycetes endoglycosidases acting on asparagine-linked oligosaccharides (2010)
  • Author(s): Satoshi Taniwaki, Tasuku Hamaguchi, Kuakarun Krusong, Jarunee Kaulpiboon, Tipaporn Limpaseni, Piamsook Pongsawasdi, and Kazuo Ito
  • Organizer: The 2nd ACP Joint Seminar
  • Place of Presentation: Khon Kaen, Thailand
  • Year and Date: 2010-11-21
• [Presentation] Mutation at subsite+2 asparagine residue of levansucrase caused the change in product size of levan (2010)
  • Author(s): Santhana Nakapong, Rath Pichyangkura, Masaru Iizuka, Kazuo Ito and Piamsook Pongsawasdi
  • Organizer: The 2nd ACP Joint Seminar
  • Place of Presentation: Khon Kaen, Thailand
  • Year and Date: 2010-11-21
• [Presentation] Characterization of amylomaltase from Thermus filiformis (2010)
  • Author(s): Kuakarun Krusong, Piriya Kaewpathomsri, Jarunee Kaulpiboon, Kazuo Ito, Shuichiro Murakami, and Piamsook Pongsawasdi
  • Organizer: The 2nd ACP Joint Seminar
  • Place of Presentation: Khon Kaen, Thailand
  • Year and Date: 2010-11-21
• [Presentation] Characterization of transglycosylation products of recombinant cyclodextringlycosyltransferase from Paenibacillus sp. BT01 (2010)
  • Author(s): Prapai Hongsa, Kazuo Ito and Tipaporn Limpaseni
  • Organizer: The 2nd ACP Joint Seminar
  • Place of Presentation: Khon Kaen, Thailand
  • Year and Date: 2010-11-21
• [Presentation] 担子菌Agaricus bisporusの産生する糖タンパク質糖鎖遊離酵素の精製とその特異性 (2010)
  • Author(s): 谷脇聡、濱口祐、Kuakarun Krusong、Jarunee Kaulpiboon、Tipaporn Limpaseni、Piamsook Pongsawasdi、伊藤和央
  • Organizer: 2010年度日本農芸化学会関西支部大会
  • Place of Presentation: 奈良
  • Year and Date: 2010-10-02
• [Presentation] 担子菌の産生するアスパラギン結合型糖鎖遊離酵素系 (2010)
  • Author(s): 濱口祐、井上裕香子、伊藤司、篠原かな子、谷脇聡、海住宜広、久田博元、秦洋二、Tipaporn Limpaseni、Piamsook Pongsawasdi、伊藤和央
  • Organizer: 第18回糖質関連酵素化学シンポジウム
  • Place of Presentation: 静岡
  • Year and Date: 2010-09-17
• [Presentation] 担子菌Agaricus bisporus由来エンド-.-N-アセチルグルコサミニダーゼABの精製とその性質 (2010)
  • Author(s): 谷脇聡、濱口祐、Kuakarun Krusong、Jarunee Kaulpiboon、Tipaporn Limpaseni、Piamsook Pongsawasdi、伊藤和央
  • Organizer: 第57回日本生化学会近畿支部例会
  • Place of Presentation: 奈良
  • Year and Date: 2010-05-22
• [Presentation] 担子菌由来ペプチドN-グリカナーゼの多様性 (2010)
  • Author(s): 篠原かな子、井上裕香子、小島美保子、濱口祐、伊藤和央
  • Organizer: 第11回関西グライコサイエンスフォーラム
  • Place of Presentation: 大阪
  • Year and Date: 2010-05-15
• [Presentation] 担子菌由来新規ペプチドN-グリカナーゼFV遺伝子のクローニングに関する研究 (2010)
  • Author(s): 篠原かな子、井上裕香子、濱口祐、Kuakarun Krusong、Jarunee Kaulpiboon、Tipaporn Limpaseni、Piamsook Pongsawasdi、伊藤和央
  • Organizer: 2010年日本農芸化学会大会
  • Place of Presentation: 東京
  • Year and Date: 2010-03-28
• [Presentation] 担子菌由来エンド-.-N-アセチルグルコサミニダーゼの発現系構築と発現酵素の性質 (2009)
  • Author(s): 濱口祐、谷脇聡、海住宜広、久田博元、秦洋二、伊藤和央
  • Organizer: 日本応用糖質科学会中国四国・近畿支部合同シンポジウム
  • Place of Presentation: 岡山
  • Year and Date: 2009-11-21
• [Presentation] 担子菌Flammulina velutipes由来ペプチドN-グリカナーゼの精製と遺伝子クローニング (2009)
  • Author(s): 濱口祐、井上裕香子、篠原かな子、谷脇聡、伊藤和央
  • Organizer: 第82回日本生化学会大会
  • Place of Presentation: 神戸
  • Year and Date: 2009-10-24
• [Presentation] 担子菌由来エンド-.-N-アセチルグルコサミニダーゼ遺伝子の発現系の構築 (2009)
  • Author(s): 濱口祐、谷脇聡、久田博元、泰洋二、伊藤和央
  • Organizer: 第56回日本生化学会近畿支部例会
  • Place of Presentation: 大阪
  • Year and Date: 2009-07-18
• [Book] Enzymatic Synthesis of Linear, Cyclic and Complex Type Oligosaccharides, Piamsook Pongsawasdiand, in Oligosaccharides : Sources, Properties and Applications Nicole S. Gordon ed (2011)
  • Author(s): Kazuo Ito
  • Total Pages: 109-134
  • Publisher: Nova Science Publishers