2011 Fiscal Year Final Research Report
Molecular analysis of anaerobic benzene degradation
| Project/Area Number |
21580118
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | Kitasato University |
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Principal Investigator |
KASAI Yuki 北里大学, 海洋バイオテクノロジー釜石研究所, 上級研究員 (20416572)
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| Project Period (FY) |
2009 – 2011
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| Keywords | 酵素化学 |
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| Research Abstract |
Intermediates formed during anaerobic benzene degradation by Azoarcus sp. DN11 was identified by gas chromatography/mass spectrometry. The data support initial methylation of benzene to toluene, followed by transformation to benzoate. Benzene-induced gene expression analysis suggested that there was a new methyltransferase gene near an anaerobic toluene degradation gene cluster. The overexpressed recombinant methyltransferase converted benzene to a metabolite that has the same retention time with toluene.
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