Molecular basis of environmental stress response mechanism mediated by plant receptor-like proteins

2011 Fiscal Year Final Research Report

• Project/Area Number: 21580125
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Bioproduction chemistry/Bioorganic chemistry
• Research Institution: The Institute of Physical and Chemical Research (2011); The University of Tokyo (2009-2010)
• Principal Investigator: OSAKABE Yuriko 独立行政法人理化学研究所, 機能開発研究グループ, 研究員 (50444071)
• Project Period (FY): 2009 – 2011
• Keywords: 受容体 / ストレス / シグナル伝達 / ペプチド

Research Abstract

Overproduction of a receptor-like kinase(RLK), RPK1, in the transgenic Arabidopsis plants enhanced tolerance to drought and oxidative stresses. Our data suggested that RPK1 functions on the adaptive mechanisms to the complex stressful environment. The cysteine-rich repeat RLK, CRK36, is a novel factor, which negatively controls the responses to osmotic stress and abscisic acid. CRK36 forms a receptor complex with a receptor-like cytosolic kinase, ARCK1, and they negatively regulate the expression of ABA-responsive transcription factors, ABI4 and ABI5.

Research Products

• [Journal Article] Abiotic stress-inducible receptor-like kinases are involved in negative regulation of ABA signaling in Arabidopsis (2012)
  • Author(s): Tanaka, H., Osakabe, Y., Katsura, S., Shinji, M., Shinozaki, K., and Yamaguchi-Shinozaki, K.
  • Journal Title: Plant J. Volume: (in press)
  • Peer Reviewed
• [Journal Article] Responses to environmental stresses in woody plants ; key to survive and longevity (2012)
  • Author(s): Osakabe, Y., Kawaoka, A., Nishikubo, N., and Osakabe K
  • Journal Title: J. Plant Research Volume: 125 Pages: 1-10
  • Peer Reviewed
• [Journal Article] The monosaccharide absorption activity of Arabidopsis roots depends on the expressional profiles of transporter genes under high-salinity condition (2011)
  • Author(s): Yamada, K., Kanai, M., Osakabe, Y., Ohiraki, H., Shinozaki, K., and Yamaguchi-Shinozaki, K.
  • Journal Title: J. Biol. Chem Volume: 286 Pages: 43577-43586
  • Peer Reviewed
• [Journal Article] Arabidopsis HsfA1 transcription factors function as the main positive regulators in heat shock-responsive gene expression (2011)
  • Author(s): Yoshida, T., Ohama, N., Nakajima, J., Kidokoro, S., Mizoi, J., Nakashima, K., Maruyama, K., Kim, J.-M., Seki, M., Todaka, D., Osakabe, Y., Sakuma, Y., Schoffl, F., Shinozaki, K., Yamaguchi-Shinozaki, K.
  • Journal Title: Mol. Genet. Genomics Volume: 286 Pages: 321-332
  • Peer Reviewed
• [Journal Article] Genetic engineering of woody plants ; current and future targets in a stressful environment (2011)
  • Author(s): Osakabe, Y., Kajita, S., Osakabe, K.
  • Journal Title: Physiologia Plantarum Volume: 142 Pages: 105-117
  • Peer Reviewed
• [Journal Article] Overproduction of a membrane-bound receptor-like protein kinase1, RPK1, enhances abiotic stress tolerance of Arabidopsis (2010)
  • Author(s): Osakabe, Y., Mizuno, S., Tanaka, H., Maruyama, K., Osakabe K, Todaka, D., Fujita, Y., Kobayashi M, Shinozaki, K., and Yamaguchi-Shinozaki, K.
  • Journal Title: J. Biol. Chem Volume: 285 Pages: 9190-9201
  • Peer Reviewed
• [Journal Article] Site-directed mutagenesis in Arabidopsis using custom-designed zinc finger nucleases (2010)
  • Author(s): Osakabe, K. Osakabe, Y., and Toki, S.
  • Journal Title: Proc. Natl. Acad. Sci. USA Volume: 107 Pages: 12034-12039
  • Peer Reviewed
• [Journal Article] RPK2 is an essential receptor-like kinase that transmits the CLV3 signal in Arabidopsis (2010)
  • Author(s): Kinoshita, A., Betsuyaku, S., Osakabe, Y., Mizuno, S., Nagawa, S., Stahl, Y., Simon, R., Yamaguchi-Shinozaki, K., Fukuda, H., and Sawa, S.
  • Journal Title: Development Volume: 137 Pages: 3911-3920
  • Peer Reviewed
• [Presentation] シロイヌナズナにおいて乾燥・高温ストレス誘導性遺伝子発現を制御する転写因子DREB2Aの新規相互作用因子NF-YC10の機能解析 (2012)
  • Author(s): 佐藤輝、溝井順哉、田中秀典、圓山恭之進、秦峰、刑部祐里子、篠崎一雄、篠崎和子
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都
  • Year and Date: 20120616-18
• [Presentation] 植物環境応答の調節と受容体型キナーゼシグナリング (2012)
  • Author(s): 刑部祐里子,田中秀典,篠崎一雄,篠崎和子
  • Organizer: 第53回日本植物生理学会年会招待講演
  • Place of Presentation: 京都
  • Year and Date: 20120316-18
• [Presentation] シロイヌナズナの水分ストレス誘導性受容体様キナーゼ遺伝子の機能解析 (2012)
  • Author(s): 田中秀典、刑部祐里子、桂彰吾、水野真二、圓山恭之進、草壁和也、溝井順哉、篠崎一雄、篠崎和子
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都
  • Year and Date: 20120316-18
• [Presentation] シロイヌナズナのストレス誘導性K+/H+交換輸送体様遺伝子AtKEA5の機能解析 (2012)
  • Author(s): 徐劭旭、刑部祐里子、田中秀典、篠崎一雄、篠崎和子
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都
  • Year and Date: 20120316-18
• [Presentation] ダイズの環境ストレス応答に関するDREB2A相同遺伝子の機能解析 (2012)
  • Author(s): 大堀鉄平、森脇崇、溝井順哉、城所聡、渡邉慶太郎、圓山恭之進、刑部祐里子、篠崎一雄、篠崎和子
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都
  • Year and Date: 20120316-18
• [Presentation] シロイヌナズナABCトランスポーターGサブファミリーの遺伝子機能解析 (2012)
  • Author(s): 大開暖香、黒森崇、刑部祐里子、山田晃嗣、桂彰吾、長町啓太、篠崎一雄、篠崎和子
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都
  • Year and Date: 20120316-18
• [Presentation] シロイヌナズナにおけるカリウムトランスポーターKUP6を介した浸透圧ストレス応答と成長制御 (2012)
  • Author(s): 刑部祐里子,桂彰吾,有永直子,長町啓太,田中秀典,山田晃嗣, Seo Souk,安保充,吉村悦郎,篠崎一雄,篠崎和子
  • Organizer: 第53回日本植物生理学会年会
  • Place of Presentation: 京都
  • Year and Date: 20120316-18
• [Presentation] Control mechanism of osmotic stress response and plant growth by potassium transporter in Arabidopsis (2011)
  • Author(s): Osakabe, Y., Katsura, S., Arinaga, N., Nagamachi, K., Tanaka, H., Yamada, K., Seo, S., Abo, M., Yoshimura, E., Shinozaki, K., Yamaguchi-Shinozaki, K.
  • Organizer: 4th International Workshop on Plant Abiotic Stress : From Systems Biology To Sustainable Agriculture
  • Place of Presentation: Limassol, Cyprus
  • Year and Date: 20111118-19
• [Presentation] Analysis of an interactor with the transcription factor DREB2A regulating water-and heat-stress-inducible gene expression in Arabidopsis (2011)
  • Author(s): Satoh, H., Mizoi, J., Tanaka, H., Qin, F., Osakabe, Y., Shinozaki, K., Yamaguchi-Shinozaki, K.
  • Organizer: 4th International Workshop on Plant Abiotic Stress : From Systems Biology To Sustainable Agriculture
  • Place of Presentation: Limassol, Cyprus
  • Year and Date: 20111118-19
• [Presentation] Functional analysis of abiotic stress-inducible genes encoding receptor-like kinases in Arabidopsis (2011)
  • Author(s): Tanaka, H., Osakabe, Y., Katsura, S., Mizuno, S., Shinozaki, K., Yamaguchi-Shinozaki, K.
  • Organizer: 12th International Symposium on Plant Protein Phosphorylation
  • Place of Presentation: Tubingen, Germany
  • Year and Date: 20110914-16
• [Presentation] Functional analysis of a rice PIF transcription factor that functions as a key regulator of internode elongation in response to drought stress (2011)
  • Author(s): Todaka, D., Nakashima, K., Ito, Y., Maruyama, K., Osakabe, Y., Fujita, Y., Matsukura, S., Yoshiwara, K., Ohme-Takagi, M., Shinozaki, K., Yamaguchi-Shinozaki, K.
  • Organizer: Plant Biology
  • Place of Presentation: Minneapolis, USA
  • Year and Date: 20110806-10
• [Presentation] 植物の受容体型キナーゼによる環境ストレス応答と化学制御 (2011)
  • Author(s): 刑部祐里子、田中秀典、篠崎一雄、篠崎和子
  • Organizer: 第84回日本生化学会大会招待講演
  • Place of Presentation: 京都
  • Year and Date: 2011-09-23
• [Presentation] ストレス耐性と受容体型キナーゼシグナル伝達調節 (2011)
  • Author(s): 刑部祐里子、田中秀典、篠崎一雄、篠崎和子
  • Organizer: 薮田セミナー
  • Place of Presentation: 東京
  • Year and Date: 2011-07-14
• [Presentation] Functional analysis of stress-induced receptor-like cytosolic kinase gene in Arabidopsis. Cold Spring Harbor Asia Conference (2010)
  • Author(s): Tanaka, H., Osakabe, Y., Katsura, S., Mizuno, S., Shinozaki, K., Yamaguchi-Shinozaki, K.
  • Organizer: From Plant Biology to Crop Biotechnology
  • Place of Presentation: Suzhou, China
  • Year and Date: 20101025-29
• [Presentation] Control mechanism of osmotic stress response and plant growth by potassium transporter in Arabidopsis (2010)
  • Author(s): Osakabe, Y., Katsura, S., Arinaga, N., Yamada, K., Tanaka, H., Seo, S., Kodaira, K., Shinozaki, K., Yamaguchi-Shinozaki, K.
  • Organizer: The 21st International Conference on Arabidopsis Research
  • Place of Presentation: Yokohama, Japan
  • Year and Date: 20100606-10
• [Presentation] シロイヌナズナの水分ストレス誘導性受容体様細胞質型キナーゼ遺伝子の機能解析 (2010)
  • Author(s): 田中秀典、刑部祐里子、桂彰吾、水野真二、篠崎一雄、篠崎和子
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 宮城
  • Year and Date: 20100320-22
• [Presentation] 茎頂分裂組織におけるCLV3シグナル受容機構の解析 (2010)
  • Author(s): 木下温子、別役重之、刑部祐里子、篠崎和子、福田裕穂、澤進一郎
  • Organizer: 第52回日本植物生理学会年会
  • Place of Presentation: 宮城
  • Year and Date: 20100320-22
• [Presentation] 植物の受容体型キナーゼによる環境ストレス応答と生長制御 (2010)
  • Author(s): 刑部祐里子
  • Organizer: 東京理科大学総合研究機構知識インターフェース研究部門講演会(招待講演)
  • Place of Presentation: 東京理科大
  • Year and Date: 2010-07-14
• [Presentation] 植物の受容体型キナーゼによる環境ストレス応答と生長制御 (2010)
  • Author(s): 刑部祐里子
  • Organizer: 農芸化学若手セミナー
  • Place of Presentation: 岡山大学
  • Year and Date: 2010-05-07
• [Presentation] シロイヌナズナの水分ストレス誘導性受容体様細胞質型キナーゼ遺伝子の機能解析 (2009)
  • Author(s): 田中秀典,刑部祐里子,桂彰吾,水野真二,篠崎一雄,篠崎和子
  • Organizer: 第32回分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 20091209-12
• [Book] 「高等植物において特定の遺伝子だけを標的として破壊する技術」化学と生物 (2011)
  • Author(s): 刑部敬史、刑部祐里子、土岐精一
  • Total Pages: 592-594
• [Book] 「人工制限酵素を利用した高等植物における標的遺伝子特異的改変技術の開発」バイオインダストリー (2011)
  • Author(s): 刑部敬史、刑部祐里子、土岐精一
  • Total Pages: 53-57