2011 Fiscal Year Final Research Report
Prediction of substrate specificity of enzymes based on sensitivity to a uniquely selective inhibitor.
| Project/Area Number |
21580128
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bioproduction chemistry/Bioorganic chemistry
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| Research Institution | Okayama University |
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Principal Investigator |
NITODA Teruhiko 岡山大学, 大学院・自然科学研究科, 准教授 (80284090)
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| Project Period (FY) |
2009 – 2011
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| Keywords | キチン / β-N-アセチルグルコサミニダーゼ / 酵素阻害剤 / TMG-chitotriomycin / 阻害選択性 / 糖質加水分解酵素ファミリー / 基質特異性 / 基質認識 |
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| Research Abstract |
β-N-Acetylhexosaminidase(β-GlcNAcase), an enzyme widely distributed in organisms, is thought to be responsible for numerous physiological processes. However, most of the physiological functions remain to be clarified. The present study revealed that the newβ-GlcNAcase inhibitor TMG-chitotriomycin had the unique selectivity based on the substrate specificity ofβ-GlcNAcase, indicating that TMG-chitotriomycin can be used as a tool for classification ofβ-GlcNAcases differ in substrate specificity and even also in physiological functions.
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