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2011 Fiscal Year Final Research Report

Relationship between the common epitope and the structure of sugar chains in plant allergens

Research Project

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Project/Area Number 21580151
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Food science
Research InstitutionOkayama Prefectural University

Principal Investigator

TSUJI Hideaki  岡山県立大学, 保健福祉学部, 教授 (20093875)

Co-Investigator(Kenkyū-buntansha) KIMOTO Masumi  岡山県立大学, 保健福祉学部, 教授 (40108866)
HIEMORI Miki  岡山県立大学, 保健福祉学部, 准教授 (80326412)
Project Period (FY) 2009 – 2011
Keywords大豆 / 小麦 / アレルゲン / 糖鎖 / 共通抗原 / 発現タンパク質 / Gly m Bd 28K / Tri a Bd 27K
Research Abstract

We investigated the allergenicity of soybean allergen, Gly m Bd 28K, and wheat allergen, Tri a Bd 27K. These allergens were shown to contain fucose and xylose in addition to mannose, N-acetyl glucosamine, indicating that the sugar moieties of these allergens may be asparagine-linmked sugar chain. Furthermore, these sugar moieties were shown to be bind IgE antibodies in sera of patients sensitive to soybean and wheat.
The cDNA encoding Gly m Bd 28K has been cloned. However, the cDNA has the part encoding the signal peptide of the precursor for Gly m Bd 28K, but the cDNA has not the perfect cDNA encoding full length of mRNA for the allergen. In the present study, three amino acid residues on the signal peputide and 20-bp on the 5'-untranlated region were elucidated by 5'-RACE kit. This finding provide useful information on the expression of the allergen in cultivated tabacco cells.

  • Research Products

    (4 results)

All 2009 Other

All Journal Article (3 results) Presentation (1 results)

  • [Journal Article] Isolation and molecular cloning of a major allergen, Tri a Bd 27K2009

    • Author(s)
      M. Kimoto, M. Suzuki, N. Komiyama, A. Kunimoto, H. Yamashita, M. Hiemori, K. Takahashi, and H. Tsuji
    • Journal Title

      Biosci. Biotech. Biochem.

      Volume: Vol.73, No.1 Pages: 85-92

  • [Journal Article] Preparation and epitope mapping of a monoclonal antibody against Tri a Bd 27K a wheat major allergen.2009

    • Author(s)
      N. Komiyama, M. Hiemori, M. Kimoto, M. Suzuki, H. Yamashita, Y. Takahashi and H. Tsuji
    • Journal Title

      Biochem. Biotechnol. Biochem

      Volume: Vol.73, No.9 Pages: 211-2116

  • [Journal Article] Investigation of multiple forms of Tri a Bd 27K a major wheat allergen, by immunoblotting analysis.

    • Author(s)
      M. Hiemori, Y. Yoshida, M. Kimoto, H. Yamashita, K. Takahashi, K. Takahashi, N. Komiyama, and H. Tsuji
    • Journal Title

      Biochem. Biotechnol. Biochem

      Volume: Vol.74, No.1 Pages: 199-202

  • [Presentation] 小麦主要アレルゲンTri a Bd 27Kに対するモノクローナル抗体の作製及びその応用2009

    • Author(s)
      小宮山展子、比江森美樹、木本眞順美、山下広美、辻英明
    • Organizer
      第63回日本栄養食糧学会本大会
    • Place of Presentation
      長崎ブリックホール
    • Year and Date
      20090500

URL: 

Published: 2013-07-31  

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