Research on correlations between the intracellular composition of fatty acids and the protein degradation machinery of insulin secretion-related enzymes.

2011 Fiscal Year Final Research Report

• Project/Area Number: 21580163
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Food science
• Research Institution: Okayama University of Science (2011); Doshisha University (2009-2010)
• Principal Investigator: ANDO Hideya 岡山理科大学, 工学部, 教授 (60454493)
• Project Period (FY): 2009 – 2011
• Keywords: 酵素 / 細胞 / 組織 / 脂質 / 蛋白質分解 / 糖尿病

Research Abstract

The effects of alterations of the intracellular composition of fatty acids on insulin secretion by cultured murine pancreatic β cells(MIN6 cells) was evaluated. The insulin secretion from MIN6 cells was enhanced most efficiently by oleic acid, followed by linoleic acid and α-linolenic acid, when each fatty acid was treated transiently at the same molar concentration. On the other hand, preincubation with each fatty acid for 72 hr in advance changed the intracellular composition of fatty acids and the most efficient effect of insulin secretion under this condition was elicited by α-linolenic acid. These results suggest that the insulin secreting activity of cultured MIN6 cells is affected by the intracellular composition of fatty acids.

Research Products

• [Journal Article] Melanosomes are transferred from melanocytes to keratinocytes through the processes of packaging, release, uptake and dispersion (2012)
  • Author(s): Ando H, Niki Y, Ito M, Akiyama K, Matsui M, Yarosh D, Ichihashi M.
  • Journal Title: Journal of Investigative Dermatology Volume: Vol.132 Pages: 1222-1229
  • Peer Reviewed
• [Journal Article] 1-(2, 4-Dihydroxyphenyl)-3-(2, 4-dimethoxy-3-methylphenyl) propane inhibits melanin synthesis by dual mechanisms (2011)
  • Author(s): Niki Y, Yoshida M, Ando H, Wakamatsu K, Ito S, Harada N, Matsui M, Yarosh D, Ichihashi M.
  • Journal Title: Journal of Dermatological Science Volume: Vol.63 Pages: 115-121
  • Peer Reviewed
• [Journal Article] Involvement of pigment globules containing multiple melanosomes in the transfer of melanosomes from melanocytes to keratinocytes (2011)
  • Author(s): Ando H, Niki Y, Yoshida M, Ito M, Akiyama K, Kim J, Yoon T, Matsui M, Yarosh D, Ichihashi M.
  • Journal Title: Cellular Logistics Volume: Vol.1 Pages: 12-20
  • Peer Reviewed
• [Journal Article] Quasi-drugs developed in Japan for the prevention or treatment of hyperpigmentary disorders (2010)
  • Author(s): Ando H, Matsui M, Ichihashi M.
  • Journal Title: International Journal of Molecular Sciences Volume: Vol.11 Pages: 2566-2575
  • Peer Reviewed
• [Journal Article] β-catenin regulates melanocyte dendricity through the modulation of PKCζand PKCδ (2010)
  • Author(s): Kim J, Sohn K, Choi T, Kim M, Ando H, Choi S, Kim S, Lee Y, Lee J, Kim C, Yoon T.
  • Journal Title: Pigment Cell and Melanoma Research Volume: Vol.23 Pages: 385-393
  • Peer Reviewed
• [Journal Article] Keratinocytes in culture accumulate phagocytosed melanosomes in the perinuclear area (2010)
  • Author(s): Ando H, Niki Y, Yoshida M, Ito M, Akiyama K, Kim J, Yoon T, Lee J, Matsui M, Ichihashi M.
  • Journal Title: Pigment Cell and Melanoma Research Volume: Vol.23 Pages: 129-133
  • Peer Reviewed
• [Journal Article] Role of the ubiquitin proteasome system in regulating skin pigmentation (2009)
  • Author(s): Ando H, Ichihashi M, Hearing V.
  • Journal Title: International Journal of Molecular Sciences Volume: Vol.10 Pages: 4428-4434
  • Peer Reviewed
• [Presentation] 紫外線による皮膚の色素沈着メカニズムについて (2011)
  • Author(s): 安藤秀哉、市橋正光
  • Organizer: 第11回日本抗加齢医学会総会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2011-05-27
• [Presentation] 培養膵β細胞のインスリン分泌作用に及ぼす長時間遊離脂肪酸処理の影響 (2011)
  • Author(s): 安藤秀哉、福島光夫、高橋良寿、今井緑、寺師浩人、清野進、市橋正光
  • Organizer: 第54回日本糖尿病学会年次学術集会
  • Place of Presentation: さっぽろ芸術文化の館
  • Year and Date: 2011-05-19
• [Presentation] Skin lightening quasi-drugs from the present into the future (2010)
  • Author(s): Ando H
  • Organizer: 6^<th> Healthy-Beauty Bio-Industry Symposium
  • Place of Presentation: Jeju Island, Korea
  • Year and Date: 2010-12-10
• [Presentation] 新規メラノソームトランスファーメカニズムの解明:多数のメラノソームを包含した膜小胞の発見 (2010)
  • Author(s): 安藤秀哉、仁木洋子、吉田雅紀、Matsui M、Yarosh D、市橋正光
  • Organizer: 第10回日本抗加齢医学会総会
  • Place of Presentation: 国立京都国際会館
  • Year and Date: 2010-06-12
• [Presentation] A new proposal for the evaluation method of melanosome transfer in respect to melanosome uptake by keratinocyte phagocytosis (2009)
  • Author(s): Ando H, Niki Y, Yoshida M, Matsui M, Ichihashi M.
  • Organizer: 22^<nd> Annual meeting of the Japanese Society for Pigment Cell Research
  • Place of Presentation: Fukuoka, Japan
  • Year and Date: 2009-12-05
• [Presentation] Inhibition of melanin synthesis via modification of tyrosinase function (2009)
  • Author(s): Ando H, Ichihashi M, Hearing VJ.
  • Organizer: 3^<rd> Conference of the Asian Society for Pigment Cell Research
  • Place of Presentation: Seoul, Korea
  • Year and Date: 2009-06-13