Effects of tetrodotoxin on the central nervous system and behavior of tiger puffer

2011 Fiscal Year Final Research Report

• Project/Area Number: 21580227
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General fisheries
• Research Institution: Nagasaki University
• Principal Investigator: SAKAKURA Yoshitaka 長崎大学, 大学院・水産・環境科学総合研究科, 教授 (20325682)
• Co-Investigator(Kenkyū-buntansha): YAMAZAKI Hideki 独立行政法人水産総合研究センター, 瀬戸内海区水産研究所, 主任技術開発員 (10466261)
• Co-Investigator(Renkei-kenkyūsha): TAKATANI Tomohiro 長崎大学, 大学院・水産・環境科学総合研究科, 准教授 (90304972)
• Project Period (FY): 2009 – 2011
• Keywords: 生理 / 行動

Research Abstract

We investigated the effects of tetrodotoxin(TTX) on the central nervous system and behavior of juvenile tiger puffer Takifugu rubripes. We compared distribution of TTX in the tissues between wild and artificially raised fish. Monoclonal anti-TTX antibody was used for detection of TTX in the various tissues of tiger puffer juveniles. We detected TTX in the skin and liver of the wild puffer juveniles. Moreover, we firstly demonstrated that TTX were present in the central nervous system, especially in the hindbrain, cerebellum, optic nerve and olfactory epithelium. When TTX was administrated to artificially raised puffer juveniles by diet and injection to muscle, the same TTX distribution as wild fish was observed. Behavioral observation revealed that artificially raised puffer juveniles, which do not contain TTX, were bold to a novel environment comparing to the wild juveniles. Administration of TTX to the juvenile puffer significantly decreased boldness and agonistic interactions, and raised the survival after release to the environment with predators. Synthesizing these results, we hypothesized that TTX is a kind of neuro-transmitter.

Research Products

• [Journal Article] III-4.フグの行動とフグ毒 (2012)
  • Author(s): 阪倉良孝、高谷智裕、山崎英樹、崎山一孝
  • Journal Title: 日本水産学会誌 Volume: Vol.78、No.1 Pages: 93
• [Journal Article] Transfer profile of intramuscularly administered tetrodotoxin to artificial hybrid specimens of pufferfishTakifugu rubripes and Takifugu niphobles (2011)
  • Author(s): Wang, J., Araki, T., Tatsuno, R., Nina, S., Ikeda, K., Hamasaki, M., Sakakura, Y., Takatani, T. & Arakawa, O.
  • Journal Title: Toxicon Volume: Vol.58, No.6-7 Pages: 565-569
  • Peer Reviewed
• [Presentation] トラフグ稚魚のフグ毒感知器官 (2012)
  • Author(s): 沖田光玄・山崎英樹・崎山一孝・山根晃・新名真也・高谷智裕・荒川修・阪倉良孝
  • Organizer: 平成24年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学,東京
  • Year and Date: 20120326-30
• [Presentation] トラフグ稚魚のフグ毒に対する選択性 (2011)
  • Author(s): 沖田光玄・中安純一・山崎英樹・崎山一孝・田中幸太郎・高谷智裕・荒川修・阪倉良孝
  • Organizer: 平成23年度日本水産学会秋期大会
  • Place of Presentation: 長崎大学,長崎
  • Year and Date: 20110928-1002
• [Presentation] Aggressive behavior and TTX in puffer fish (2011)
  • Author(s): Sakakura, Y., Takatani, T., Nakayasu, J., Okita, K., Yamasaki, H., Sakiyama, K.
  • Organizer: ICCPB 2011
  • Place of Presentation: Nagoya, Japan
  • Year and Date: 20110531-0605
• [Presentation] トラフグ天然稚魚とフグ毒投与人工種苗の組織内でのフグ毒蓄積部位の比較 (2011)
  • Author(s): 沖田光玄・中安純一・山崎英樹・崎山一孝・田中幸太郎・高谷智裕・荒川修・阪倉良孝
  • Organizer: 平成23年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学,東京
  • Year and Date: 20110327-31
• [Presentation] フグの行動とフグ毒 (2011)
  • Author(s): 阪倉良孝
  • Organizer: 平成23年度日本水産学会秋期大会シンポジウム「フグ研究とトラフグ生産技術開発の最前線」
  • Place of Presentation: 長崎大学,長崎
  • Year and Date: 2011-10-02
• [Presentation] 捕食者の提示がトラフグ種苗の行動に与える影響 (2009)
  • Author(s): 中安純一・山崎英樹・崎山一孝・福島優喜・角南慶卓・佐藤哲哉・阪倉良孝・高谷智裕
  • Organizer: 平成21年度日本水産学会秋季大会
  • Place of Presentation: いわて県民情報交流センター,盛岡
  • Year and Date: 20090930-1003
• [Presentation] フグの行動とフグ毒 (2009)
  • Author(s): 阪倉良孝・山崎英樹・崎山一孝
  • Organizer: 平成21年度日本水産学会九州支部例会シンポジウム～九州発フグ研究と生産技術開発の最前線～
  • Place of Presentation: 長崎大学水産学部,長崎
  • Year and Date: 2009-09-19