Virus detection and prevention of diseases brought by black tiger prawn migrating toward north with global warming

2011 Fiscal Year Final Research Report

• Project/Area Number: 21580230
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General fisheries
• Research Institution: University of Miyazaki
• Principal Investigator: ITAMI Toshiaki 宮崎大学, 農学部, 教授 (00363573)
• Co-Investigator(Kenkyū-buntansha): YOSHIDA Terutoyo 宮崎大学, 農学部, 准教授 (20240294)
• Project Period (FY): 2009 – 2011
• Keywords: 魚病 / クルマエビ / ウシエビ / ウイルス / 検出 / RNAi

Research Abstract

Expanding the global warming, black tiger prawn, Penaeus monodon, has been migrating toward the north. In Hyuganada sea are, native species of kuruma prawn, Marsupenaeus japonicas, and up-coming tiger prawn are occupying the same habitat. In order to detect the virus that might have been brought by black tiger prawn, a sensitive detection method has been established. Cutting-edge technologies for preventing diseases have been attempted.
1. Pathogenic viruses have not been detected in the black tiger prawn found in Hyuganada sea are using real-time LAMP method.
2. Any virus-positive black tiger prawn was not found along the Miyazaki cost line.
3. Shrimp showed the resistance against WSSV infection when caspase gene was knocked down.

Research Products

• [Journal Article] Class B scavenger receptor, Croquemort from kuruma shrimp Marsupenaeus japonicus (2011)
  • Author(s): Mekata, T., Okugawa, S., Inada, M., Yoshimine, M., Nishi, J., Kono, T., Sakai, M., Sudhakaran, R, Itami, T
  • Journal Title: Molecular cloning and characterization Volume: 25(2-3) Pages: 94-100
  • Peer Reviewed
• [Journal Article] A novel gene of tumor necrosis factor ligand superfamily from kuruma shrimp Marsupenaeus japonicus (2010)
  • Author(s): Mekata, T., Sudhakaran, R., Okugawa, S., Inada, M., Kono, T., Sakai, M., Itami, T
  • Journal Title: Fish and Shellfish Immunology Volume: 28-2 Pages: 571-578
  • Peer Reviewed
• [Journal Article] Molecular cloning and characterization of the nitric oxide synthase gene from kuruma shrimp Marsupenaeus japonicus (2010)
  • Author(s): Inada, M., Mekata, T., Sudhakaran, R., Okugawa, S., Kono, T., El Asely, A. M., Linh, N., Yoshida, T., Sakai, M., Yui, T., Itami, T.
  • Journal Title: Fish and Shellfish Immunology Volume: 28-3 Pages: 701-710
  • Peer Reviewed
• [Journal Article] Molecular cloning and transcriptional analysis of a newly identified anti-lipopolysaccharide factor gene in kuruma shrimp, Marsupenaeus japonicus (2010)
  • Author(s): Mekata, T., Sudhakaran, R., Okugawa, S., Kono, T., Sakai, M., Itami, T
  • Journal Title: Letters in Applied Microbiology Volume: 50-1 Pages: 112-119
  • Peer Reviewed
• [Journal Article] Real-time transcription loop-mediated isothermal amplification for rapid detection of yellow head virus in shrimp (2009)
  • Author(s): Mekata, T., Sudhakaran, R., Kono, T., U-taynapun, K., Supamattaya, K., Suzuki, Y., Sakai, M., Itami, T
  • Journal Title: Journal of Virological Methods Volume: 162-1 Pages: 81-87
  • Peer Reviewed
• [Presentation] Identification and Expression Analysis of Toll interacting protein gene (2011)
  • Author(s): K. Kihara, M. Yoshimine, M. Inada, J. Nishi, T. Yoshida, T. Kono, M. Sakai and T. Itami
  • Organizer: 8th Symposium on Diseases in Asian Aquaculture
  • Place of Presentation: Mangalore, India
  • Year and Date: 2011-11-23
• [Presentation] Identification and Expression Analysis of Astakine Gene (2011)
  • Author(s): M Inada, S Okugawa. J Nishi, M Yoshimine, R Sudhakaran, T Kono, T Mekata, T Yoshida, M Sakai and T Itami
  • Organizer: Plant and Animal Genome XIX Conference
  • Place of Presentation: San Diego, USA
  • Year and Date: 2011-01-19
• [Presentation] Identification and Characterization of Dicer2 Gene in Kuruma Shrimp (2010)
  • Author(s): J Nishi, S Okugawa, M Inada, M Yoshimine, T Kono, T Mekata, T Yoshida, M Sakai, and T Itami
  • Organizer: The 39th Scientific Symposium of UJNR Aquaculture Panel
  • Place of Presentation: Kagoshima, Japan
  • Year and Date: 2010-10-26
• [Presentation] Development and Evaluation of Real-time Loop-mediated Isothermal Amplifications Assay for Detection of Disease Viruses in Penaeid Shrimp (2010)
  • Author(s): T Itami, T Mekata, R Sudhakaran, T Kono, T Yoshida, Y Suzuki, K Supamattaya M Inada, S Okugawa, J Nishi, M Yoshimine and M Sakai
  • Organizer: 6th International Symposium on Aquatic Animal Health
  • Place of Presentation: Tampa, USA
  • Year and Date: 2010-09-07
• [Presentation] Characterization of Tumor Necrosis Factor Ligand Superfamily Gene in Kuruma Shrimp (2010)
  • Author(s): S Okugawa, R Sudhakaran, M Inada, T Mekata, J Nishi, M Yoshimine, T Yoshida, Y Suzuki, T Kono, M Sakai and T Itami
  • Organizer: 6th International Symposium on Aquatic Animal Health
  • Place of Presentation: Tampa, USA
  • Year and Date: 2010-09-07
• [Book] 第3章ウイルス病§6甲殻類などにおけるウイルス病、「改定・魚病学概論」 (2012)
  • Author(s): 伊丹利明
  • Total Pages: 49-52
  • Publisher: 恒星社厚生閣(東京)
• [Remarks]
  • URL: http://www.agr.miyazaki-u.ac.jp/～fishery/staff/staff07/
• [Patent(Industrial Property Rights)] LAMP法による急性ウイルス血症原因ウイルスの検出方法及び検出試薬キット (2011)
  • Inventor(s): 伊丹利明,酒井正博,河野智哉,国立大学法人宮崎大学
  • Industrial Property Rights Holder: 国立大学法人宮崎大学
  • Industrial Property Number: 特許、特願2011-50110
  • Filing Date: 2011-03-08
• [Patent(Industrial Property Rights)] 甲殻類急性ウイルス血症に対するワクチン (2010)
  • Inventor(s): 酒井正博,伊丹利明,河野智哉,国立大学法人宮崎大学
  • Industrial Property Rights Holder: 国立大学法人宮崎大学
  • Industrial Property Number: 特許、PCT/JP2010/060503
  • Filing Date: 2010-06-22
  • Overseas
• [Patent(Industrial Property Rights)] 甲殻類急性ウイルス血症に対するワクチン (2009)
  • Inventor(s): 酒井正博,伊丹利明,河野智哉,国立大学法人宮崎大学
  • Industrial Property Rights Holder: 国立大学法人宮崎大学
  • Industrial Property Number: 特許、特願2009-162802
  • Filing Date: 2009-07-09