2011 Fiscal Year Final Research Report
The molecular basis of bovine colostrum kappa casein for inhibitory mechanism against human rotavirus infection
| Project/Area Number |
21580325
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Zootechnical science/Grassland science
|
|---|
| Research Institution | Gifu University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2009 – 2011
|
| Keywords | カッパカゼイン / ロタウイルス / 牛乳 / 糖鎖 / 腸管感染症 / 食品 |
|---|
| Research Abstract |
Human rotavirus (HRV) is a major etiologic agent of severe infantile gastroenteritis. In this study, we investigated the inhibitory effect of bovine kappa casein (к-CN) against HRV infection. к-CN is the only casein characterized with O-linked glycans. Deglycosylated casein obtained by neuraminidase and O-glycosidase treatments did not exhibit the anti-HRV activity, suggesting that O-linked glycan structure in к-CN is essential for its antivirus activity. From the analysis of MALDI-TOF MS, the composition of к-CN glycan was different between mature milk and late colostrum obtained from normal cows at 6-7 day after parturition. Experimentally indicated slightly higher activity of k-CN from the latter than that from the former could be ascribed to the difference in the attached carbohydrates.
|
