2011 Fiscal Year Final Research Report
Development of a general method for obtaining double KO swine cells which will be used as donors for production of cloned homozygous piglets
| Project/Area Number |
21580350
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied animal science
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| Research Institution | Kagoshima University |
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Principal Investigator |
SATO Masahiro 鹿児島大学, フロンティアサイエンス研究推進センター, 教授 (30287099)
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| Co-Investigator(Kenkyū-buntansha) |
YOSHIDA Mitsutoshi 鹿児島大学, 農学部, 教授 (00174954)
MIYOSHI Kazuchika 鹿児島大学, 農学部, 准教授 (70363611)
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| Project Period (FY) |
2009 – 2011
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| Keywords | KOブタ / ホモ / gene targeting / 薬剤耐性 / 体細胞核移植 / 胎仔性繊維芽細胞 / 遺伝子導入 / double KO |
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| Research Abstract |
This study aims to develop a general method for obtaining double KO swine cels in which the target gene expression is completely blocked. This was successfully performed by selection of heterogenous KO cels under higher concentrations of selective drugs after repeated delivery of a targeting vector(RDTV). These resulting double KO cells will be used for somatic cell nuclear transfer-mediated production of cloned pigs.
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